Identification of a chicken RAD52 homologue suggests conservation of the RAD52 recombination pathway throughout the evolution of higher eukaryotes

Olga Y. Bezzubova; Henning Schmidt; Kai Ostermann; Wolf Dietrich Heyer; Jean Marie Buerstedde

Identification of a chicken RAD52 homologue suggests conservation of the RAD52 recombination pathway throughout the evolution of higher eukaryotes

Olga Y. Bezzubova, Henning Schmidt, Kai Ostermann, Wolf Dietrich Heyer, Jean Marie Buerstedde

Research output: Contribution to journal › Article › peer-review

Abstract

Degenerate oligonucleotides encoding conserved regions of the Rad52 protein of S.cerevisiae and its homologue, the Rad22 protein of S.pombe, were used to clone a chicken RAD52 counterpart by the polymerase chain reaction. Sequence comparison of the chicken and yeast proteins reveals a strongly conserved region between positions 40 and 178 of the chicken Rad52 sequence indicating that this part of the protein is under strong evolutionary pressure. The first 39 amino acids and the 3′ end of the chicken Rad52 homologue does not share significant similarity with the yeast proteins. High abundance of the mRNA in testis makes it likely that the chicken Rad52 protein plays a role in meiotic recombination.

Original language	English (US)
Pages (from-to)	5945-5949
Number of pages	5
Journal	Nucleic Acids Research
Volume	21
Issue number	25
State	Published - Dec 25 1993
Externally published	Yes

ASJC Scopus subject areas

Genetics
Statistics, Probability and Uncertainty
Applied Mathematics
Health, Toxicology and Mutagenesis
Toxicology
Genetics(clinical)

Cite this

Identification of a chicken RAD52 homologue suggests conservation of the RAD52 recombination pathway throughout the evolution of higher eukaryotes. / Bezzubova, Olga Y.; Schmidt, Henning; Ostermann, Kai; Heyer, Wolf Dietrich; Buerstedde, Jean Marie.

In: Nucleic Acids Research, Vol. 21, No. 25, 25.12.1993, p. 5945-5949.

Research output: Contribution to journal › Article › peer-review

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abstract = "Degenerate oligonucleotides encoding conserved regions of the Rad52 protein of S.cerevisiae and its homologue, the Rad22 protein of S.pombe, were used to clone a chicken RAD52 counterpart by the polymerase chain reaction. Sequence comparison of the chicken and yeast proteins reveals a strongly conserved region between positions 40 and 178 of the chicken Rad52 sequence indicating that this part of the protein is under strong evolutionary pressure. The first 39 amino acids and the 3′ end of the chicken Rad52 homologue does not share significant similarity with the yeast proteins. High abundance of the mRNA in testis makes it likely that the chicken Rad52 protein plays a role in meiotic recombination.",

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T1 - Identification of a chicken RAD52 homologue suggests conservation of the RAD52 recombination pathway throughout the evolution of higher eukaryotes

AU - Bezzubova, Olga Y.

AU - Schmidt, Henning

AU - Ostermann, Kai

AU - Heyer, Wolf Dietrich

AU - Buerstedde, Jean Marie

PY - 1993/12/25

Y1 - 1993/12/25

N2 - Degenerate oligonucleotides encoding conserved regions of the Rad52 protein of S.cerevisiae and its homologue, the Rad22 protein of S.pombe, were used to clone a chicken RAD52 counterpart by the polymerase chain reaction. Sequence comparison of the chicken and yeast proteins reveals a strongly conserved region between positions 40 and 178 of the chicken Rad52 sequence indicating that this part of the protein is under strong evolutionary pressure. The first 39 amino acids and the 3′ end of the chicken Rad52 homologue does not share significant similarity with the yeast proteins. High abundance of the mRNA in testis makes it likely that the chicken Rad52 protein plays a role in meiotic recombination.

AB - Degenerate oligonucleotides encoding conserved regions of the Rad52 protein of S.cerevisiae and its homologue, the Rad22 protein of S.pombe, were used to clone a chicken RAD52 counterpart by the polymerase chain reaction. Sequence comparison of the chicken and yeast proteins reveals a strongly conserved region between positions 40 and 178 of the chicken Rad52 sequence indicating that this part of the protein is under strong evolutionary pressure. The first 39 amino acids and the 3′ end of the chicken Rad52 homologue does not share significant similarity with the yeast proteins. High abundance of the mRNA in testis makes it likely that the chicken Rad52 protein plays a role in meiotic recombination.

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Identification of a chicken RAD52 homologue suggests conservation of the RAD52 recombination pathway throughout the evolution of higher eukaryotes

Abstract

ASJC Scopus subject areas

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