Identification of a chicken RAD52 homologue suggests conservation of the RAD52 recombination pathway throughout the evolution of higher eukaryotes

Olga Y. Bezzubova, Henning Schmidt, Kai Ostermann, Wolf Dietrich Heyer, Jean Marie Buerstedde

Research output: Contribution to journalArticlepeer-review

66 Scopus citations

Abstract

Degenerate oligonucleotides encoding conserved regions of the Rad52 protein of S.cerevisiae and its homologue, the Rad22 protein of S.pombe, were used to clone a chicken RAD52 counterpart by the polymerase chain reaction. Sequence comparison of the chicken and yeast proteins reveals a strongly conserved region between positions 40 and 178 of the chicken Rad52 sequence indicating that this part of the protein is under strong evolutionary pressure. The first 39 amino acids and the 3′ end of the chicken Rad52 homologue does not share significant similarity with the yeast proteins. High abundance of the mRNA in testis makes it likely that the chicken Rad52 protein plays a role in meiotic recombination.

Original languageEnglish (US)
Pages (from-to)5945-5949
Number of pages5
JournalNucleic Acids Research
Volume21
Issue number25
StatePublished - Dec 25 1993
Externally publishedYes

ASJC Scopus subject areas

  • Genetics
  • Statistics, Probability and Uncertainty
  • Applied Mathematics
  • Health, Toxicology and Mutagenesis
  • Toxicology
  • Genetics(clinical)

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