Identification of a bone matrix-derived chemotactic factor

M. Somerman, A. T. Hewitt, H. H. Varner, E. Schiffmann, J. Termine, A Hari Reddi

Research output: Contribution to journalArticlepeer-review

44 Scopus citations

Abstract

When demineralized bone matrix powder is implanted subcutaneously in the rat, the early responses involve the appearance and proliferation of mesenchymal cells at the site of implantation, followed by cartilage and bone formation. The ability of cells to migrate to the implant suggests that chemotaxis may be a critical event in this process. Therefore, using the modified Boyden chamber assay, we tested extracts of demineralized bone matrix for chemotactic activity. We have identified and partially purified, on molecular sieve chromatography, a heat labile and trypsin-sensitive protein (Mr=60,000-70,000) that is a potent chemoattractant for mouse calvaria, osteoblast-like cells (MMB-1), but not for monocytes (putative osteoclast precursors). These findings suggest that chemotactic protein(s) have a significant role in the recruitment of osteoprogenitor cells to a site of bone repair.

Original languageEnglish (US)
Pages (from-to)481-485
Number of pages5
JournalCalcified Tissue International
Volume35
Issue number1
DOIs
StatePublished - Dec 1983
Externally publishedYes

Keywords

  • Bone
  • Bone proteins
  • Chemotaxis
  • Osteoblasts

ASJC Scopus subject areas

  • Endocrinology

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