Identification of 14-3-3ε substrates from embryonic murine brain

Bryan A. Ballif; Zhongwei Cao; Daniel Schwartz; Kermit L Carraway; Steven P. Gygi

doi:10.1021/pr060206k

Identification of 14-3-3ε substrates from embryonic murine brain

Bryan A. Ballif, Zhongwei Cao, Daniel Schwartz, Kermit L Carraway, Steven P. Gygi

Biochemistry and Molecular Medicine

Research output: Contribution to journal › Article › peer-review

41 Scopus citations

Abstract

Mice deficient in 14-3-3ε exhibit abnormal neuronal migration and die perinatally. We report here the first large-scale analysis of 14-3-3 interacting partners from primary animal tissue, identifying from embryonic murine brain 163 14-3-3ε interacting proteins and 85 phosphorylation sites on these proteins. Phosphorylation of the deubiquitinating enzyme USP8 at serine 680 was found essential for its interaction with 14-3-3ε and for maintaining USP8 in the cytosol.

Original language	English (US)
Pages (from-to)	2372-2379
Number of pages	8
Journal	Journal of Proteome Research
Volume	5
Issue number	9
DOIs	https://doi.org/10.1021/pr060206k
State	Published - Sep 2006

Keywords

14-3-3
Brain development
Deubiquitination
Epsilon
Kinase
Mass spectrometry
Phosphoproteomics
Phosphorylation

ASJC Scopus subject areas

Genetics
Biotechnology
Biochemistry

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abstract = "Mice deficient in 14-3-3ε exhibit abnormal neuronal migration and die perinatally. We report here the first large-scale analysis of 14-3-3 interacting partners from primary animal tissue, identifying from embryonic murine brain 163 14-3-3ε interacting proteins and 85 phosphorylation sites on these proteins. Phosphorylation of the deubiquitinating enzyme USP8 at serine 680 was found essential for its interaction with 14-3-3ε and for maintaining USP8 in the cytosol.",

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T1 - Identification of 14-3-3ε substrates from embryonic murine brain

AU - Ballif, Bryan A.

AU - Cao, Zhongwei

AU - Schwartz, Daniel

AU - Carraway, Kermit L

AU - Gygi, Steven P.

PY - 2006/9

Y1 - 2006/9

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AB - Mice deficient in 14-3-3ε exhibit abnormal neuronal migration and die perinatally. We report here the first large-scale analysis of 14-3-3 interacting partners from primary animal tissue, identifying from embryonic murine brain 163 14-3-3ε interacting proteins and 85 phosphorylation sites on these proteins. Phosphorylation of the deubiquitinating enzyme USP8 at serine 680 was found essential for its interaction with 14-3-3ε and for maintaining USP8 in the cytosol.

KW - 14-3-3

KW - Brain development

KW - Deubiquitination

KW - Epsilon

KW - Kinase

KW - Mass spectrometry

KW - Phosphoproteomics

KW - Phosphorylation

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