Abstract
Mice deficient in 14-3-3ε exhibit abnormal neuronal migration and die perinatally. We report here the first large-scale analysis of 14-3-3 interacting partners from primary animal tissue, identifying from embryonic murine brain 163 14-3-3ε interacting proteins and 85 phosphorylation sites on these proteins. Phosphorylation of the deubiquitinating enzyme USP8 at serine 680 was found essential for its interaction with 14-3-3ε and for maintaining USP8 in the cytosol.
Original language | English (US) |
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Pages (from-to) | 2372-2379 |
Number of pages | 8 |
Journal | Journal of Proteome Research |
Volume | 5 |
Issue number | 9 |
DOIs | |
State | Published - Sep 2006 |
Keywords
- 14-3-3
- Brain development
- Deubiquitination
- Epsilon
- Kinase
- Mass spectrometry
- Phosphoproteomics
- Phosphorylation
ASJC Scopus subject areas
- Genetics
- Biotechnology
- Biochemistry