Identification of 14-3-3ε substrates from embryonic murine brain

Bryan A. Ballif, Zhongwei Cao, Daniel Schwartz, Kermit L Carraway, Steven P. Gygi

Research output: Contribution to journalArticle

40 Scopus citations

Abstract

Mice deficient in 14-3-3ε exhibit abnormal neuronal migration and die perinatally. We report here the first large-scale analysis of 14-3-3 interacting partners from primary animal tissue, identifying from embryonic murine brain 163 14-3-3ε interacting proteins and 85 phosphorylation sites on these proteins. Phosphorylation of the deubiquitinating enzyme USP8 at serine 680 was found essential for its interaction with 14-3-3ε and for maintaining USP8 in the cytosol.

Original languageEnglish (US)
Pages (from-to)2372-2379
Number of pages8
JournalJournal of Proteome Research
Volume5
Issue number9
DOIs
StatePublished - Sep 2006

Keywords

  • 14-3-3
  • Brain development
  • Deubiquitination
  • Epsilon
  • Kinase
  • Mass spectrometry
  • Phosphoproteomics
  • Phosphorylation

ASJC Scopus subject areas

  • Genetics
  • Biotechnology
  • Biochemistry

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