Abstract
Mice deficient in 14-3-3ε exhibit abnormal neuronal migration and die perinatally. We report here the first large-scale analysis of 14-3-3 interacting partners from primary animal tissue, identifying from embryonic murine brain 163 14-3-3ε interacting proteins and 85 phosphorylation sites on these proteins. Phosphorylation of the deubiquitinating enzyme USP8 at serine 680 was found essential for its interaction with 14-3-3ε and for maintaining USP8 in the cytosol.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 2372-2379 |
| Number of pages | 8 |
| Journal | Journal of Proteome Research |
| Volume | 5 |
| Issue number | 9 |
| DOIs | |
| State | Published - Sep 2006 |
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Keywords
- 14-3-3
- Brain development
- Deubiquitination
- Epsilon
- Kinase
- Mass spectrometry
- Phosphoproteomics
- Phosphorylation
ASJC Scopus subject areas
- Genetics
- Biotechnology
- Biochemistry
Cite this
Identification of 14-3-3ε substrates from embryonic murine brain. / Ballif, Bryan A.; Cao, Zhongwei; Schwartz, Daniel; Carraway, Kermit L; Gygi, Steven P.
In: Journal of Proteome Research, Vol. 5, No. 9, 09.2006, p. 2372-2379.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Identification of 14-3-3ε substrates from embryonic murine brain
AU - Ballif, Bryan A.
AU - Cao, Zhongwei
AU - Schwartz, Daniel
AU - Carraway, Kermit L
AU - Gygi, Steven P.
PY - 2006/9
Y1 - 2006/9
N2 - Mice deficient in 14-3-3ε exhibit abnormal neuronal migration and die perinatally. We report here the first large-scale analysis of 14-3-3 interacting partners from primary animal tissue, identifying from embryonic murine brain 163 14-3-3ε interacting proteins and 85 phosphorylation sites on these proteins. Phosphorylation of the deubiquitinating enzyme USP8 at serine 680 was found essential for its interaction with 14-3-3ε and for maintaining USP8 in the cytosol.
AB - Mice deficient in 14-3-3ε exhibit abnormal neuronal migration and die perinatally. We report here the first large-scale analysis of 14-3-3 interacting partners from primary animal tissue, identifying from embryonic murine brain 163 14-3-3ε interacting proteins and 85 phosphorylation sites on these proteins. Phosphorylation of the deubiquitinating enzyme USP8 at serine 680 was found essential for its interaction with 14-3-3ε and for maintaining USP8 in the cytosol.
KW - 14-3-3
KW - Brain development
KW - Deubiquitination
KW - Epsilon
KW - Kinase
KW - Mass spectrometry
KW - Phosphoproteomics
KW - Phosphorylation
UR - http://www.scopus.com/inward/record.url?scp=33748325681&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=33748325681&partnerID=8YFLogxK
U2 - 10.1021/pr060206k
DO - 10.1021/pr060206k
M3 - Article
C2 - 16944949
AN - SCOPUS:33748325681
VL - 5
SP - 2372
EP - 2379
JO - Journal of Proteome Research
JF - Journal of Proteome Research
SN - 1535-3893
IS - 9
ER -