Identification of 12-lipoxygenase in the hemolymph of tiger shrimp (Penaeus japonicus Bate)

Jen Min Kuo, Bonnie Sun Pan, Hongjian Zhang, J. Bruce German

Research output: Contribution to journalArticlepeer-review

14 Scopus citations


Lipoxygenase (LOX) activity was found in the hemolymph of live tiger shrimp (Penaues japonicus Bate) but not in the muscle, branchiae, midgut gland, eye, brain, and spinal cord. Oxygen consumption rate of shrimp hemolymph catalyzed oxidation of arachidonic acid was 1.32 nmol min-1 (mg of protein)-1. The product was determined to be 12-hydroxyeicosatetraenoic acid (12-HETE) on the basis of retention time in HPLC analysis. The 12-HETE was found to have a conjugated structure on the basis of UV absorbance spectroscopy and to be oxygenated at the C-12 position on the basis of mass spectroscopy and HPLC analysis. The amount of 12-HETE was proportional to oxygen consumption. A 12-lipoxygenase (LOX) activity was identified in the hemolymph of shrimp. This was the first observation demonstrating LOX activity in a marine crustacean.

Original languageEnglish (US)
Pages (from-to)1620-1623
Number of pages4
JournalJournal of Agricultural and Food Chemistry
Issue number8
StatePublished - 1994


  • 12-HETE
  • Hemolymph
  • Lipoxygenase
  • Shrimp

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Food Science
  • Chemistry (miscellaneous)


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