Identification of 12 EST-derived SSR markers in Lumbricus rubellus

Zachary Lounsberry, Mark C. Ungerer, Bruce A. Snyder

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

While many species of earthworms are globally distributed, very little is known about the genetic population dynamics of this diverse group. We present the characterization of novel simple sequence repeat (SSR) markers, including primer information, number of alleles, repeat motif, and approximate size ranges, to be used in population genetic analyses of the earthworm Lumbricus rubellus Hoffmeister 1843. Specifically, we designed and characterized 12 novel, polymorphic markers derived from published expressed sequence tags (EST) for amplification in L. rubellus. The mean number of alleles per locus was 6.25 ± 1.91, indicating these markers will be sufficiently polymorphic for population genetic studies of this species.

Original languageEnglish (US)
Pages (from-to)191-193
Number of pages3
JournalPedobiologia
Volume56
Issue number4-6
DOIs
StatePublished - Jul 1 2013

Fingerprint

Lumbricus rubellus
expressed sequence tags
earthworms
population genetics
microsatellite repeats
alleles
earthworm
allele
population dynamics
range size
loci
amplification
marker

Keywords

  • Earthworm
  • Invasion ecology
  • Population genetics
  • Simple sequence repeats

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Soil Science

Cite this

Identification of 12 EST-derived SSR markers in Lumbricus rubellus. / Lounsberry, Zachary; Ungerer, Mark C.; Snyder, Bruce A.

In: Pedobiologia, Vol. 56, No. 4-6, 01.07.2013, p. 191-193.

Research output: Contribution to journalArticle

Lounsberry, Zachary ; Ungerer, Mark C. ; Snyder, Bruce A. / Identification of 12 EST-derived SSR markers in Lumbricus rubellus. In: Pedobiologia. 2013 ; Vol. 56, No. 4-6. pp. 191-193.
@article{b62bac51efec400ab5d34af190beeb5a,
title = "Identification of 12 EST-derived SSR markers in Lumbricus rubellus",
abstract = "While many species of earthworms are globally distributed, very little is known about the genetic population dynamics of this diverse group. We present the characterization of novel simple sequence repeat (SSR) markers, including primer information, number of alleles, repeat motif, and approximate size ranges, to be used in population genetic analyses of the earthworm Lumbricus rubellus Hoffmeister 1843. Specifically, we designed and characterized 12 novel, polymorphic markers derived from published expressed sequence tags (EST) for amplification in L. rubellus. The mean number of alleles per locus was 6.25 ± 1.91, indicating these markers will be sufficiently polymorphic for population genetic studies of this species.",
keywords = "Earthworm, Invasion ecology, Population genetics, Simple sequence repeats",
author = "Zachary Lounsberry and Ungerer, {Mark C.} and Snyder, {Bruce A.}",
year = "2013",
month = "7",
day = "1",
doi = "10.1016/j.pedobi.2013.07.002",
language = "English (US)",
volume = "56",
pages = "191--193",
journal = "Pedobiologia",
issn = "0031-4056",
publisher = "Urban und Fischer Verlag Jena",
number = "4-6",

}

TY - JOUR

T1 - Identification of 12 EST-derived SSR markers in Lumbricus rubellus

AU - Lounsberry, Zachary

AU - Ungerer, Mark C.

AU - Snyder, Bruce A.

PY - 2013/7/1

Y1 - 2013/7/1

N2 - While many species of earthworms are globally distributed, very little is known about the genetic population dynamics of this diverse group. We present the characterization of novel simple sequence repeat (SSR) markers, including primer information, number of alleles, repeat motif, and approximate size ranges, to be used in population genetic analyses of the earthworm Lumbricus rubellus Hoffmeister 1843. Specifically, we designed and characterized 12 novel, polymorphic markers derived from published expressed sequence tags (EST) for amplification in L. rubellus. The mean number of alleles per locus was 6.25 ± 1.91, indicating these markers will be sufficiently polymorphic for population genetic studies of this species.

AB - While many species of earthworms are globally distributed, very little is known about the genetic population dynamics of this diverse group. We present the characterization of novel simple sequence repeat (SSR) markers, including primer information, number of alleles, repeat motif, and approximate size ranges, to be used in population genetic analyses of the earthworm Lumbricus rubellus Hoffmeister 1843. Specifically, we designed and characterized 12 novel, polymorphic markers derived from published expressed sequence tags (EST) for amplification in L. rubellus. The mean number of alleles per locus was 6.25 ± 1.91, indicating these markers will be sufficiently polymorphic for population genetic studies of this species.

KW - Earthworm

KW - Invasion ecology

KW - Population genetics

KW - Simple sequence repeats

UR - http://www.scopus.com/inward/record.url?scp=84887989628&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84887989628&partnerID=8YFLogxK

U2 - 10.1016/j.pedobi.2013.07.002

DO - 10.1016/j.pedobi.2013.07.002

M3 - Article

AN - SCOPUS:84887989628

VL - 56

SP - 191

EP - 193

JO - Pedobiologia

JF - Pedobiologia

SN - 0031-4056

IS - 4-6

ER -