Identification and structural elucidation of lectin-binding oligosaccharides by bioaffinity matrix-assisted laser desorption/ionization fourier transform mass spectrometry

K. Tseng, H. Wang, Carlito B Lebrilla, B. Bonnell, J. Hedrick

Research output: Contribution to journalArticle

13 Scopus citations

Abstract

Cortical granule lectin (CGL) is released by the egg of the South African toad Xenopus laevis upon fertilization. The lectin binds to oligosaccharides in the extracellular matrix of the egg to form a physical block to prevent additional sperm penetration or polyspermy. To identify the oligosaccharides that bind to CGL, the lectin was immobilized on the surface of a matrix-assisted laser desorption/ionization probe. This bioaffmity probe was used to determine oligosaccharides that bind preferentially to CGL. Structural analyses based on collision-induced dissociation was used to determine that oligosaccharides with the sulfate esters at the nonreducing ends preferentially bind to the lectin.

Original languageEnglish (US)
Pages (from-to)3556-3561
Number of pages6
JournalAnalytical Chemistry
Volume73
Issue number15
DOIs
StatePublished - Aug 1 2001

ASJC Scopus subject areas

  • Analytical Chemistry

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