Identification and sequence of the drpA gene from Escherichia coli

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14 Scopus citations

Abstract

The drpA gene of Escherichia coli encodes a factor that is involved in global RNA synthesis. We establish that the drpA gene has been successfully cloned and describe the fine-structure map of three drpA-(Ts) mutations as well as the complete nucleotide sequence of the drpA gene. We identified a major sigma-70 promoter for the drpA gene on the bases of (i) its similarity to the consensus sequence and (ii) S1 protection and primer extension mapping data. In addition, the nucleotide sequence revealed a pair of dnaA boxes and a factor-independent terminator at the 5' end and 3' end of the gene, respectively. The deduced amino acid sequence of the DrpA protein showed a nucleotide-binding pocket found in some ATPases.

Original languageEnglish (US)
Pages (from-to)281-286
Number of pages6
JournalJournal of Bacteriology
Volume172
Issue number1
StatePublished - 1990

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Immunology

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