Identification and sequence analysis of lpfABCDE, a putative fimbrial operon of Salmonella typhimurium

Andreas J Baumler, F. Heffron

Research output: Contribution to journalArticle

131 Citations (Scopus)

Abstract

A chromosomal region present in Salmonella typhimurium but absent from related species was identified by hybridization. A DNA probe originating from 78 min on the S. typhimurium chromosome hybridized with DNA from Salmonella enteritidis, Salmonella heidelberg, and Salmonella dublin but not with DNA from Salmonella typhi, Salmonella arizonae, Escherichia coli, and Shigella serotypes. Cloning and sequence analysis revealed that the corresponding region of the S. typhimurium chromosome encodes a fimbrial operon. Long fimbriae inserted at the poles of the bacterium were observed by electron microscopy when this fimbrial operon was introduced into a nonpiliated E. coli strain. The genes encoding these fimbriae were therefore termed lpfABCDE, for long polar fimbriae. Genetically, the lpf operon was found to be most closely related to the fim operon of S. typhimurium, both in gene order and in conservation of the deduced amino acid sequences.

Original languageEnglish (US)
Pages (from-to)2087-2097
Number of pages11
JournalJournal of Bacteriology
Volume177
Issue number8
StatePublished - 1995
Externally publishedYes

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Salmonella typhimurium
Operon
Sequence Analysis
Salmonella
Salmonella arizonae
Chromosomes
Escherichia coli
Salmonella enteritidis
Salmonella typhi
Shigella
Gene Order
DNA
DNA Probes
Organism Cloning
Amino Acid Sequence
Electron Microscopy
Bacteria
Genes

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Immunology

Cite this

Identification and sequence analysis of lpfABCDE, a putative fimbrial operon of Salmonella typhimurium. / Baumler, Andreas J; Heffron, F.

In: Journal of Bacteriology, Vol. 177, No. 8, 1995, p. 2087-2097.

Research output: Contribution to journalArticle

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