Identification and partial characterization of an endogenous form of mouse mammary tumor virus that is transcribed into the virion-associated RNA genome

T. G. Fanning, J. P. Puma, Robert Cardiff

Research output: Contribution to journalArticle

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Abstract

Restriction mapping demonstrated the presence of several distinct proviral forms of mouse mammary tumor virus in the genome of GR mice. One of these proviruses (GR-MTV-2) was highly amplified in GR 3A cells, a cell line derived from a GR mammary tumor. By the criterion of restriction mapping, the amplified GR-MTV-2 provirus found in GR 3A cells was identical to the provirus found in Ml.19 cells, a rat cell line infected with virions obtained from GR 3A culture fluid. This result clearly implies that the GR-MTV-2 provirus in GR 3A cells was transcribed into the virion-associated viral RNA genome. Cleavage of either GR 3A or Ml.19 cell DNAs with the restriction enzyme Bgl II gave rise to a 2.6×106 dalton GR-MTV-2 proviral fragment (ca. 45% of the viral genome). This fragment was isolated and mapped with thirteen restriction enzymes.

Original languageEnglish (US)
Pages (from-to)5715-5723
Number of pages9
JournalNucleic Acids Research
Volume8
Issue number23
DOIs
StatePublished - Dec 11 1980

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Mouse mammary tumor virus
Proviruses
Virion
Genome
RNA
Restriction Mapping
Viral Genome
Cell Line
DNA Restriction Enzymes
Viral RNA
Breast Neoplasms
Enzymes

ASJC Scopus subject areas

  • Genetics

Cite this

Identification and partial characterization of an endogenous form of mouse mammary tumor virus that is transcribed into the virion-associated RNA genome. / Fanning, T. G.; Puma, J. P.; Cardiff, Robert.

In: Nucleic Acids Research, Vol. 8, No. 23, 11.12.1980, p. 5715-5723.

Research output: Contribution to journalArticle

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