Identification and optimization of DNA aptamer binding regions using DNA microarrays.

Nicholas O Fischer, Theodore M. Tarasow

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

DNA aptamers are versatile recognition elements for pharmaceutical, diagnostic, and life science applications. Identification and optimization of the minimal functional sequence after aptamer selection is a bottleneck for developing aptamer applications. DNA microarray technology proved a facile means for screening thousands of aptamer sequence permutations to identify functional aptamer domains. This chapter describes the detailed methodology for designing aptamer arrays to identify minimal aptamer binding domains as well as elucidating the relationship between aptamer structure and function, using immunoglobulin E as a model protein.

Original languageEnglish (US)
Pages (from-to)57-66
Number of pages10
JournalMethods in molecular biology (Clifton, N.J.)
Volume723
DOIs
StatePublished - 2011
Externally publishedYes

Fingerprint

Nucleotide Aptamers
Biological Science Disciplines
Oligonucleotide Array Sequence Analysis
Immunoglobulin E
Technology
Pharmaceutical Preparations
Proteins

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Identification and optimization of DNA aptamer binding regions using DNA microarrays. / Fischer, Nicholas O; Tarasow, Theodore M.

In: Methods in molecular biology (Clifton, N.J.), Vol. 723, 2011, p. 57-66.

Research output: Contribution to journalArticle

@article{9533bf398ac446608e088bc569374bc3,
title = "Identification and optimization of DNA aptamer binding regions using DNA microarrays.",
abstract = "DNA aptamers are versatile recognition elements for pharmaceutical, diagnostic, and life science applications. Identification and optimization of the minimal functional sequence after aptamer selection is a bottleneck for developing aptamer applications. DNA microarray technology proved a facile means for screening thousands of aptamer sequence permutations to identify functional aptamer domains. This chapter describes the detailed methodology for designing aptamer arrays to identify minimal aptamer binding domains as well as elucidating the relationship between aptamer structure and function, using immunoglobulin E as a model protein.",
author = "Fischer, {Nicholas O} and Tarasow, {Theodore M.}",
year = "2011",
doi = "10.1007/978-1-61779-043-0_5",
language = "English (US)",
volume = "723",
pages = "57--66",
journal = "Methods in molecular biology (Clifton, N.J.)",
issn = "1064-3745",
publisher = "Humana Press",

}

TY - JOUR

T1 - Identification and optimization of DNA aptamer binding regions using DNA microarrays.

AU - Fischer, Nicholas O

AU - Tarasow, Theodore M.

PY - 2011

Y1 - 2011

N2 - DNA aptamers are versatile recognition elements for pharmaceutical, diagnostic, and life science applications. Identification and optimization of the minimal functional sequence after aptamer selection is a bottleneck for developing aptamer applications. DNA microarray technology proved a facile means for screening thousands of aptamer sequence permutations to identify functional aptamer domains. This chapter describes the detailed methodology for designing aptamer arrays to identify minimal aptamer binding domains as well as elucidating the relationship between aptamer structure and function, using immunoglobulin E as a model protein.

AB - DNA aptamers are versatile recognition elements for pharmaceutical, diagnostic, and life science applications. Identification and optimization of the minimal functional sequence after aptamer selection is a bottleneck for developing aptamer applications. DNA microarray technology proved a facile means for screening thousands of aptamer sequence permutations to identify functional aptamer domains. This chapter describes the detailed methodology for designing aptamer arrays to identify minimal aptamer binding domains as well as elucidating the relationship between aptamer structure and function, using immunoglobulin E as a model protein.

UR - http://www.scopus.com/inward/record.url?scp=79958283258&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=79958283258&partnerID=8YFLogxK

U2 - 10.1007/978-1-61779-043-0_5

DO - 10.1007/978-1-61779-043-0_5

M3 - Article

VL - 723

SP - 57

EP - 66

JO - Methods in molecular biology (Clifton, N.J.)

JF - Methods in molecular biology (Clifton, N.J.)

SN - 1064-3745

ER -