Identification and isolation of a 155-kDa protein with neuropathy target esterase activity

Christopher E. Mackay, Bruce D. Hammock, Barry W. Wilson

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

A method is presented for the isolation of a 155-kDa protein that possesses phenyl valerate hydrolysis activity in the presence of paraoxon but is inhibited by mipafox; the functional definition of neuropathy target esterase (neurotoxic esterase; NTE). Microsomes, isolated from 18-day-old chicken embryos were treated with phospholipase A2 to solubilize the NTE activity. The extract was then combined with polyoxyethylene W1 detergent and resolved by gel filtration chromatography to yield an active fraction with an approximate mass of 200 kDa. This fraction was further purified by preparative isoelectric focusing and native electrophoresis to yield two separate bands possessing NTE activity. The slower migrating band was highly enriched in a 155-kDa protein that was identified as a source of the NTE activity by affinity chromatography using 3-(9′-mercaptononylthio)-1,1,1-trifluoro-propan-2-one bound to Sepharose CL6B. This represents the first report of the isolation of NTE in its active form and aids in the confirmation of the 155-kDa protein as the most likely candidate for NTE.

Original languageEnglish (US)
Article number0039
Pages (from-to)23-30
Number of pages8
JournalToxicological Sciences
Volume30
Issue number1
DOIs
StatePublished - 1996

Fingerprint

Paraoxon
Affinity chromatography
Proteins
Phospholipases A2
Isoelectric Focusing
Microsomes
Chromatography
Electrophoresis
Affinity Chromatography
Detergents
Sepharose
Gel Chromatography
Chickens
Hydrolysis
Embryonic Structures
Gels
neurotoxic esterase
mipafox
phenyl valerate

ASJC Scopus subject areas

  • Toxicology

Cite this

Identification and isolation of a 155-kDa protein with neuropathy target esterase activity. / Mackay, Christopher E.; Hammock, Bruce D.; Wilson, Barry W.

In: Toxicological Sciences, Vol. 30, No. 1, 0039, 1996, p. 23-30.

Research output: Contribution to journalArticle

Mackay, Christopher E. ; Hammock, Bruce D. ; Wilson, Barry W. / Identification and isolation of a 155-kDa protein with neuropathy target esterase activity. In: Toxicological Sciences. 1996 ; Vol. 30, No. 1. pp. 23-30.
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