Identification and DNA sequence analysis of the Marek's disease virus serotype 2 genes homologous to the herpes simplex virus type 1 UL20 and UL21

Shinichi Hatama, Hyung Kwan Jang, Yoshihiro Izumiya, Jin Shun Cai, Yoshinori Tsushima, Kentaro Kato, Takayuki Miyazawa, Chieko Kai, Eiji Takahashi, Takeshi Mikami

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

We determined 3,135 bp of the nucleotide sequence located in an 8.5 kb EcoRI-E fragment in the unique long (UL) genome region of Marek's disease virus serotype 2 (MDV2), and identified UL20 and UL21 homologous genes of herpes simplex virus type 1 (HSV-1). The UL20 and UL21 homologous genes of MDV2 are arranged colinearly with the prototype sequence of HSV-1. In addition, an open reading frame (MDV2 ORF 273), which has been identified within the UL21 homologous gene of MDV2, has no apparent relation to any other known herpesvirus genes. Northern blot analysis and reverse transcriptase polymerase chain reaction confirmed the existance of RNA transcripts related to the UL20 and ORF 273 genes in MDV2-infected cells, except no transcript related to the UL21 gene being detected. The putative protein product of the MDV2 UL20 gene had a relatively low homology but that of the MDV2 UL21 gene had a moderate homology among herpesviruses. Further, the possible functions and features of the predicted proteins encoded within the sequenced region are discussed.

Original languageEnglish (US)
Pages (from-to)587-593
Number of pages7
JournalJournal of Veterinary Medical Science
Volume61
Issue number6
StatePublished - Jun 1999
Externally publishedYes

Fingerprint

Gallid Herpesvirus 3
Human herpesvirus 1
Mardivirus
Human Herpesvirus 1
DNA Sequence Analysis
serotypes
sequence analysis
nucleotide sequences
Genes
genes
Open Reading Frames
open reading frames
Herpesviridae
Marek's disease virus serotype 2
Gene
Sequence analysis
Virus
protein products
messenger RNA
Reverse Transcriptase Polymerase Chain Reaction

Keywords

  • Marek's disease virus serotype 2
  • Nucleotide sequence
  • ORF 273
  • UL20
  • UL21

ASJC Scopus subject areas

  • veterinary(all)
  • Marketing

Cite this

Identification and DNA sequence analysis of the Marek's disease virus serotype 2 genes homologous to the herpes simplex virus type 1 UL20 and UL21. / Hatama, Shinichi; Jang, Hyung Kwan; Izumiya, Yoshihiro; Cai, Jin Shun; Tsushima, Yoshinori; Kato, Kentaro; Miyazawa, Takayuki; Kai, Chieko; Takahashi, Eiji; Mikami, Takeshi.

In: Journal of Veterinary Medical Science, Vol. 61, No. 6, 06.1999, p. 587-593.

Research output: Contribution to journalArticle

Hatama, S, Jang, HK, Izumiya, Y, Cai, JS, Tsushima, Y, Kato, K, Miyazawa, T, Kai, C, Takahashi, E & Mikami, T 1999, 'Identification and DNA sequence analysis of the Marek's disease virus serotype 2 genes homologous to the herpes simplex virus type 1 UL20 and UL21', Journal of Veterinary Medical Science, vol. 61, no. 6, pp. 587-593.
Hatama, Shinichi ; Jang, Hyung Kwan ; Izumiya, Yoshihiro ; Cai, Jin Shun ; Tsushima, Yoshinori ; Kato, Kentaro ; Miyazawa, Takayuki ; Kai, Chieko ; Takahashi, Eiji ; Mikami, Takeshi. / Identification and DNA sequence analysis of the Marek's disease virus serotype 2 genes homologous to the herpes simplex virus type 1 UL20 and UL21. In: Journal of Veterinary Medical Science. 1999 ; Vol. 61, No. 6. pp. 587-593.
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