TY - JOUR
T1 - Identification and characterization of undifferentiated mast cells in mouse bone marrow
AU - Jamur, Maria Célia
AU - Grodzki, Ana
AU - Berenstein, Elsa H.
AU - Hamawy, Majed M.
AU - Siraganian, Reuben P.
AU - Oliver, Constance
PY - 2005/6/1
Y1 - 2005/6/1
N2 - Sequential immunomagnetic isolation with 2 monoclonal antibodies was used to purify and characterize an undifferentiated mast cell in adult mouse bone marrow that had not been previously recognized. This cell represents 0.02% of the cells in the bone marrow, is CD34+, CD13+, and c-kit+, and does not express FcεRI. However, by polymerase chain reaction (PCR) the cell contains message for the α and β subunits of FcεRI, mast cell-specific proteases, and carboxypeptidase A. Morphologically, this cell has a large nucleus, little cytoplasm, few cytoplasmic organelles, and no cytoplasmic granules. In vitro, in the presence of interleukin-3 (IL-3) and stem cell factor (SCF) these cells differentiate only into a granulated mast cell that now expresses CD13, c-kit, mast cell-specific gangliosides, FcεRI, and binds immunoglobulin E (IgE). When injected into lethally irradiated mice, these cells are able to reconstitute the mast cell population in the spleen.
AB - Sequential immunomagnetic isolation with 2 monoclonal antibodies was used to purify and characterize an undifferentiated mast cell in adult mouse bone marrow that had not been previously recognized. This cell represents 0.02% of the cells in the bone marrow, is CD34+, CD13+, and c-kit+, and does not express FcεRI. However, by polymerase chain reaction (PCR) the cell contains message for the α and β subunits of FcεRI, mast cell-specific proteases, and carboxypeptidase A. Morphologically, this cell has a large nucleus, little cytoplasm, few cytoplasmic organelles, and no cytoplasmic granules. In vitro, in the presence of interleukin-3 (IL-3) and stem cell factor (SCF) these cells differentiate only into a granulated mast cell that now expresses CD13, c-kit, mast cell-specific gangliosides, FcεRI, and binds immunoglobulin E (IgE). When injected into lethally irradiated mice, these cells are able to reconstitute the mast cell population in the spleen.
UR - http://www.scopus.com/inward/record.url?scp=19344373224&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=19344373224&partnerID=8YFLogxK
U2 - 10.1182/blood-2004-02-0756
DO - 10.1182/blood-2004-02-0756
M3 - Article
C2 - 15718418
AN - SCOPUS:19344373224
VL - 105
SP - 4282
EP - 4289
JO - Blood
JF - Blood
SN - 0006-4971
IS - 11
ER -