Identification and characterization of homeobox transcription factor genes in Strongylocentrotus purpuratus, and their expression in embryonic development

Meredith Howard-Ashby, Stefan C. Materna, Charles Brown, Lili Chen, R. Andrew Cameron, Eric H. Davidson

Research output: Contribution to journalArticle

97 Citations (Scopus)

Abstract

A set of 96 homeobox transcription factors was identified in the Strongylocentrotus purpuratus genome using permissive blast searches with a large collection of authentic homeodomain sequences from mouse, human and fly. A phylogenetic tree was constructed to compare the sea urchin homeobox gene family to those of vertebrates, with the result that with the only a few exceptions, orthologs of all vertebrate homeodomain genes were uncovered by our search. QPCR time course measurements revealed that 65% of these genes are expressed within the first 48 h of development (late gastrula). For genes displaying sufficiently high levels of transcript during the first 24 h of development (late blastula), whole mount in situ hybridization was carried out up to 48 h to determine spatial patterns of expression. The results demonstrate that homeodomain transcription factors participate in multiple and diverse developmental functions, in that they are used at a range of time points and in every territory of the developing embryo.

Original languageEnglish (US)
Pages (from-to)74-89
Number of pages16
JournalDevelopmental Biology
Volume300
Issue number1
DOIs
StatePublished - Dec 1 2006
Externally publishedYes

Fingerprint

Strongylocentrotus purpuratus
Homeobox Genes
Embryonic Development
Transcription Factors
Vertebrates
Genes
Blastula
Gastrula
Sea Urchins
Diptera
In Situ Hybridization
Embryonic Structures
Genome

Keywords

  • Development
  • Homeobox
  • Sea urchin
  • Transcription factor

ASJC Scopus subject areas

  • Molecular Biology
  • Developmental Biology
  • Cell Biology

Cite this

Identification and characterization of homeobox transcription factor genes in Strongylocentrotus purpuratus, and their expression in embryonic development. / Howard-Ashby, Meredith; Materna, Stefan C.; Brown, Charles; Chen, Lili; Cameron, R. Andrew; Davidson, Eric H.

In: Developmental Biology, Vol. 300, No. 1, 01.12.2006, p. 74-89.

Research output: Contribution to journalArticle

Howard-Ashby, Meredith ; Materna, Stefan C. ; Brown, Charles ; Chen, Lili ; Cameron, R. Andrew ; Davidson, Eric H. / Identification and characterization of homeobox transcription factor genes in Strongylocentrotus purpuratus, and their expression in embryonic development. In: Developmental Biology. 2006 ; Vol. 300, No. 1. pp. 74-89.
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