Identification and characterization of complete RTX operons in Moraxella bovoculi and Moraxella ovis

John A. Angelos, Louise M. Ball, John F. Hess

Research output: Contribution to journalArticlepeer-review

28 Scopus citations


To determine if Moraxella bovoculi (M. bovoculi), a recently characterized coccoid Moraxella that was isolated from the eyes of calves affected with infectious bovine keratoconjunctivitis (IBK), and Moraxella ovis (M. ovis), originally isolated from sheep with conjunctivitis, possessed genes encoding RTX proteins, genomic DNA was amplified with oligonucleotide primers targeting RTX operon genes of Moraxella bovis (M. bovis). Complete classical RTX operons composed of RTXCABD genes closely linked to a putative secretion accessory protein encoding gene (tolC) were identified in M. bovoculi and M. ovis and were designated mbvCABDtolC and movCABDtolC, respectively. These genes were closely related to M. bovis mbxCABDtolC. Polyclonal rabbit antiserum against the carboxy terminus of M. bovoculi MbvA neutralized hemolytic activity of both M. bovoculi and M. ovis; this antiserum did not neutralize the hemolytic activity of M. bovis. M. bovoculi and M. ovis possess genes that encode proteins related to pathogenic factors of M. bovis.

Original languageEnglish (US)
Pages (from-to)73-79
Number of pages7
JournalVeterinary Microbiology
Issue number1-2
StatePublished - Nov 15 2007
Externally publishedYes


  • Cattle
  • Cytotoxin
  • Hemolysin
  • IBK
  • Infectious bovine keratoconjunctivitis
  • Moraxella bovis
  • Moraxella bovoculi
  • Moraxella ovis
  • RTX operon
  • RTX toxin

ASJC Scopus subject areas

  • Animal Science and Zoology
  • Microbiology
  • veterinary(all)


Dive into the research topics of 'Identification and characterization of complete RTX operons in Moraxella bovoculi and Moraxella ovis'. Together they form a unique fingerprint.

Cite this