Identification and characterization of a novel synthetic peptide substrate specific for Src-family protein tyrosine kinases

Kit Lam, J. Wu, Q. Lou

Research output: Contribution to journalArticle

76 Scopus citations

Abstract

Using a random combinatorial peptide library method [Wu, J., Ma, Q. N. and Lam, K. S. (1994) Biochemistry 33, 14825-148333 a novel peptide, YIYGSFK, was identified as a substrate for p60(c-Src) protein tyrosine kinase. Mass spectrometric analysis showed that tyrosine-3 from the N-terminus was the phosphorylation site. Kinetic studies showed that the K(m) of YIYGSFK for p60(c-Src) was 55 μM, about 6.4-fold lower than a peptide derived from p34(cdc2) [cdc2(6-20), KVEKIGEGTYGVVYK], which had been reported to be a specific and efficient substrate for the Src-family protein tyrosine kinases. Comparison of the specificity of YIYGSFK and cdc2(6-20) as a substrate for various Src-family and non-Src-family protein tyrosine kinases suggests that YIYGSFK is a much more specific and efficient substrate for the Src-family protein tyrosine kinases.

Original languageEnglish (US)
Pages (from-to)587-592
Number of pages6
JournalInternational Journal of Peptide and Protein Research
Volume45
Issue number6
StatePublished - 1995
Externally publishedYes

Keywords

  • Combinatorial library
  • Peptide substances
  • Protein tyrosine kinases
  • Substrate specificity

ASJC Scopus subject areas

  • Biochemistry

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