Identification and characterization of a novel alpaca respiratory coronavirus most closely related to the human coronavirus 229E

Beate Crossley, Richard E. Mock, Scott A. Callison, Sharon K. Hietala

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

In 2007, a novel coronavirus associated with an acute respiratory disease in alpacas (Alpaca Coronavirus, ACoV) was isolated. Full-length genomic sequencing of the ACoV demonstrated the genome to be consistent with other Alphacoronaviruses. A putative additional open-reading frame was identified between the nucleocapsid gene and 3'UTR. The ACoV was genetically most similar to the common human coronavirus (HCoV) 229E with 92.2% nucleotide identity over the entire genome. A comparison of spike gene sequences from ACoV and from HCoV-229E isolates recovered over a span of five decades showed the ACoV to be most similar to viruses isolated in the 1960's to early 1980's. The true origin of the ACoV is unknown, however a common ancestor between the ACoV and HCoV-229E appears to have existed prior to the 1960's, suggesting virus transmission, either as a zoonosis or anthroponosis, has occurred between alpacas and humans.

Original languageEnglish (US)
Pages (from-to)3689-3700
Number of pages12
JournalViruses
Volume4
Issue number12
DOIs
StatePublished - 2012

Fingerprint

Human Coronavirus 229E
New World Camelids
Coronavirus
Genome
Viruses
Nucleocapsid
Zoonoses
3' Untranslated Regions
Acute Disease
Open Reading Frames
Genes
Nucleotides

Keywords

  • Alpaca
  • Anthroponosis
  • Coronavirus
  • Human
  • Molecular evolution
  • Respiratory
  • Reverse zoonosis

ASJC Scopus subject areas

  • Infectious Diseases
  • Virology

Cite this

Identification and characterization of a novel alpaca respiratory coronavirus most closely related to the human coronavirus 229E. / Crossley, Beate; Mock, Richard E.; Callison, Scott A.; Hietala, Sharon K.

In: Viruses, Vol. 4, No. 12, 2012, p. 3689-3700.

Research output: Contribution to journalArticle

Crossley, Beate ; Mock, Richard E. ; Callison, Scott A. ; Hietala, Sharon K. / Identification and characterization of a novel alpaca respiratory coronavirus most closely related to the human coronavirus 229E. In: Viruses. 2012 ; Vol. 4, No. 12. pp. 3689-3700.
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