Identification and characterization of a glycosyltransferase involved in acinetobacter baumannii lipopolysaccharide core biosynthesis

Nicole R. Luke, Shauna L. Sauberan, Thomas A. Russo, Janet M. Beanan, Ruth Olson, Thomas W Loehfelm, Andrew D. Cox, F. St Michael, Evgeny V. Vinogradov, Anthony A. Campagnari

Research output: Contribution to journalArticle

55 Citations (Scopus)

Abstract

Although Acinetobacter baumannii has emerged as a significant cause of nosocomial infections worldwide, there have been few investigations describing the factors important for A. baumannii persistence and pathogenesis. This paper describes the first reported identification of a glycosyltransferase, LpsB, involved in lipopolysaccharide (LPS) biosynthesis in A. baumannii. Mutational, structural, and complementation analyses indicated that LpsB is a core oligosaccharide glycosyl transferase. Using a genetic approach, lpsB was compared with the lpsB homologues of several A. baumannii strains. These analyses indicated that LpsB is highly conserved among A. baumannii isolates. Furthermore, we developed a monoclonal antibody, monoclonal antibody 13C11, which reacts to an LPS core epitope expressed by approximately one-third of the A. baumannii clinical isolates evaluated to date. Previous studies describing the heterogeneity of A. baumannii LPS were limited primarily to structural analyses; therefore, studies evaluating the correlation between these surface glycolipids and pathogenesis were warranted. Our data from an evaluation of LpsB mutant 307::TN17, which expresses a deeply truncated LPS glycoform consisting of only two 3-deoxy-D-manno-octulosonic acid residues and lipid A, suggest that A. baumannii LPS is important for resistance to normal human serum and confers a competitive advantage for survival in vivo. These results have important implications for the role of LPS in A. baumannii infections.

Original languageEnglish (US)
Pages (from-to)2017-2023
Number of pages7
JournalInfection and Immunity
Volume78
Issue number5
DOIs
StatePublished - May 2010
Externally publishedYes

Fingerprint

Acinetobacter baumannii
Glycosyltransferases
Lipopolysaccharides
Acinetobacter Infections
Monoclonal Antibodies
Lipid A
Glycolipids
Cross Infection
Epitopes
Survival

ASJC Scopus subject areas

  • Immunology
  • Microbiology
  • Parasitology
  • Infectious Diseases

Cite this

Identification and characterization of a glycosyltransferase involved in acinetobacter baumannii lipopolysaccharide core biosynthesis. / Luke, Nicole R.; Sauberan, Shauna L.; Russo, Thomas A.; Beanan, Janet M.; Olson, Ruth; Loehfelm, Thomas W; Cox, Andrew D.; Michael, F. St; Vinogradov, Evgeny V.; Campagnari, Anthony A.

In: Infection and Immunity, Vol. 78, No. 5, 05.2010, p. 2017-2023.

Research output: Contribution to journalArticle

Luke, NR, Sauberan, SL, Russo, TA, Beanan, JM, Olson, R, Loehfelm, TW, Cox, AD, Michael, FS, Vinogradov, EV & Campagnari, AA 2010, 'Identification and characterization of a glycosyltransferase involved in acinetobacter baumannii lipopolysaccharide core biosynthesis', Infection and Immunity, vol. 78, no. 5, pp. 2017-2023. https://doi.org/10.1128/IAI.00016-10
Luke, Nicole R. ; Sauberan, Shauna L. ; Russo, Thomas A. ; Beanan, Janet M. ; Olson, Ruth ; Loehfelm, Thomas W ; Cox, Andrew D. ; Michael, F. St ; Vinogradov, Evgeny V. ; Campagnari, Anthony A. / Identification and characterization of a glycosyltransferase involved in acinetobacter baumannii lipopolysaccharide core biosynthesis. In: Infection and Immunity. 2010 ; Vol. 78, No. 5. pp. 2017-2023.
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