Identification and characterization of a fish natural resistance-associated macrophage protein (NRAMP) cDNA

Jeroen Saeij, Geert F. Wiegertjes, René J M Stet

Research output: Contribution to journalArticlepeer-review

32 Scopus citations

Abstract

The mouse Lsh/Ity/Bcg locus regulates natural resistance to intracellular pathogens, and the Nramp1 gene was isolated as its candidate. Nramp is part of a small family of at least two genes, Nramp1 and Nramp2. In the present study, a full-length cDNA for carp NRAMP has been isolated and characterized. Nucleotide and predicted amino acid sequence analysis indicate that the carp NRAMP encodes a 548 amino acid membrane protein with 12 putative transmembrane domains, two N-linked glycosylation sites, and an evolutionarily conserved consensus transport motif. The peptide sequence identity among carp and human NRAMP2 is 78%, and 65% with human NRAMP1. Reverse transcription-polymerase chain reaction revealed that carp NRAMP is ubiquitously expressed. Phylogenetic analysis, using neigbor-joining, showed that the carp NRAMP protein clustered together with mammalian NRAMP2 proteins.

Original languageEnglish (US)
Pages (from-to)60-66
Number of pages7
JournalImmunogenetics
Volume50
Issue number1-2
DOIs
StatePublished - 1999
Externally publishedYes

Keywords

  • Carp
  • Evolution
  • Expression
  • Fish
  • NRAMP

ASJC Scopus subject areas

  • Immunology
  • Genetics

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