Abstract
An arachidonic acid 15-lipoxygenase activity was discovered in the gill tissue of teleost fishes during purification of the previously recognized and more preponderant 12-lipoxygenase enzyme. The total activity of this enzyme following purification using hydroxylapatite was significantly greater than in the crude tissue preparation, suggesting that an inhibition was removed during purification. The enzyme was active toward polyunsaturated fatty acids present in the tissue producing hydroxylated metabolites from fatty acids with 18-, 20-, and 22-carbon chain lengths at carbons 13, 15, and 17, respectively The enzyme was further purified by using a Superose gel filtration column and eluted with an apparent molecular weight of 70 000.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 2144-2147 |
| Number of pages | 4 |
| Journal | Journal of Agricultural and Food Chemistry |
| Volume | 38 |
| Issue number | 12 |
| State | Published - 1990 |
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ASJC Scopus subject areas
- Agricultural and Biological Sciences (miscellaneous)
- Food Science
- Chemistry (miscellaneous)
Cite this
Identification and characterization of a 15-lipoxygenase from fish gills. / German, J. Bruce; Creveling, Richard K.
In: Journal of Agricultural and Food Chemistry, Vol. 38, No. 12, 1990, p. 2144-2147.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Identification and characterization of a 15-lipoxygenase from fish gills
AU - German, J. Bruce
AU - Creveling, Richard K.
PY - 1990
Y1 - 1990
N2 - An arachidonic acid 15-lipoxygenase activity was discovered in the gill tissue of teleost fishes during purification of the previously recognized and more preponderant 12-lipoxygenase enzyme. The total activity of this enzyme following purification using hydroxylapatite was significantly greater than in the crude tissue preparation, suggesting that an inhibition was removed during purification. The enzyme was active toward polyunsaturated fatty acids present in the tissue producing hydroxylated metabolites from fatty acids with 18-, 20-, and 22-carbon chain lengths at carbons 13, 15, and 17, respectively The enzyme was further purified by using a Superose gel filtration column and eluted with an apparent molecular weight of 70 000.
AB - An arachidonic acid 15-lipoxygenase activity was discovered in the gill tissue of teleost fishes during purification of the previously recognized and more preponderant 12-lipoxygenase enzyme. The total activity of this enzyme following purification using hydroxylapatite was significantly greater than in the crude tissue preparation, suggesting that an inhibition was removed during purification. The enzyme was active toward polyunsaturated fatty acids present in the tissue producing hydroxylated metabolites from fatty acids with 18-, 20-, and 22-carbon chain lengths at carbons 13, 15, and 17, respectively The enzyme was further purified by using a Superose gel filtration column and eluted with an apparent molecular weight of 70 000.
UR - http://www.scopus.com/inward/record.url?scp=0038949027&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0038949027&partnerID=8YFLogxK
M3 - Article
AN - SCOPUS:0038949027
VL - 38
SP - 2144
EP - 2147
JO - Journal of Agricultural and Food Chemistry
JF - Journal of Agricultural and Food Chemistry
SN - 0021-8561
IS - 12
ER -