Id-1 is not expressed in the luminal epithelial cells of mammary glands

Norihisa Uehara, Yu Chien Chou, Jose J. Galvez, Paola de-Candia, Robert Cardiff, Robert Benezra, Gopalan Shyamala

Research output: Contribution to journalArticlepeer-review

17 Scopus citations

Abstract

Background: The family of inhibitor of differentiation/DNA binding (Id) proteins is known to regulate development in several tissues. One member of this gene family, Id-1, has been implicated in mammary development and carcinogenesis. Mammary glands contain various cell types, among which the luminal epithelial cells are primarily targeted for proliferation, differentiation and carcinogenesis. Therefore, to assess the precise significance of Id-1 in mammary biology and carcinogenesis, we examined its cellular localization in vivo using immunohistochemistry. Methods: Extracts of whole mammary glands from wild type and Id-1 null mutant mice, and tissue sections from paraffin-embedded mouse mammary glands from various developmental stages and normal human breast were subjected to immunoblot and immunohistochemical analyses, respectively. In both these procedures, an anti-Id-1 rabbit polyclonal antibody was used for detection of Id-1. Results: In immunoblot analyses, using whole mammary gland extracts, Id-1 was detected. In immunohistochemical analyses, however, Id-1 was not detected in the luminal epithelial cells of mammary glands during any stage of development, but it was detected in vascular endothelial cells. Conclusion: Id-1 is not expressed in the luminal epithelial cells of mammary glands.

Original languageEnglish (US)
Article numberR25
JournalBreast Cancer Research
Volume5
Issue number2
DOIs
StatePublished - Apr 1 2003

Keywords

  • Human
  • Id-1
  • Immunohistochemistry
  • Mammary glands
  • Mouse

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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