Hypothermia prevents increased capillary permeability following ischemia-reperfusion injury

Gregory Jurkovich, Roger M. Pitt, P. William Curreri, D. Neil Granger

Research output: Contribution to journalArticle

73 Citations (Scopus)

Abstract

Severely injured trauma victims are frequently hypothermic. It is nuclear, however, whether hypothermia itself is a detrimental or protective physiologic response to injury. One of the major consequences of fluid resuscitation following ischemic injury is edema formation, characterized by ischemia-reperfusion injury models. The purpose of this study was to examine the effect of regional hypothermia on a feline intestinal model of ischemia-reperfusion injury. An autoperfused segment of cat ileum was isolated and arterial, venous, and lymphatic vessels were cannulated. Lymph flow (Ql), lymph (C1), and plasma (Cp) protein concentrations and segmental blood flow (Qb) were measured. Permeability changes were characterized by the minimal Cl Cp ratio obtained by elevating venous outflow pressure. Animals were divided into the following groups: Group I: 1 hr of intestinal ischemia (30 mm Hg) with autoreperfusion; Group II: 1 hr of intestinal hypothermia (28°C) with subsequent rewarming; Group III: 1 hr of combined ischemia and hypothermia. Group III animals were either kept hypothermic (IIIA) or rewarmed (IIIB) during autoreperfusion. Minimal Cl Cp ratios (mean ± SEM) were as follows: Control: 0.15 ± 0.02; Group I*: 0.32 ± 0.03; Group II: 0.15 ± 0.01; Group IIIA: 0.18 ± 0.02; Group IIIB*: 0.42 ± 0.02; (* = P < 0.01 vs control). Reperfusion flow rates were no different between Group IIIA and Group IIIB animals. Ischemia-reperfusion, but not hypothermia alone, caused a marked increase in intestinal capillary permeability. Permeability increased after combined ischemia and hypothermia only if reperfusion was accompanied by rewarming. Hypothermic reperfusion protected against the increased permeability following ischemia.

Original languageEnglish (US)
Pages (from-to)514-521
Number of pages8
JournalJournal of Surgical Research
Volume44
Issue number5
DOIs
StatePublished - Jan 1 1988
Externally publishedYes

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Capillary Permeability
Hypothermia
Reperfusion Injury
Ischemia
Reperfusion
Rewarming
Permeability
Lymph
Wounds and Injuries
Lymphatic Vessels
Venous Pressure
Felidae
Ileum
Resuscitation
Blood Proteins
Edema
Cats

ASJC Scopus subject areas

  • Surgery

Cite this

Hypothermia prevents increased capillary permeability following ischemia-reperfusion injury. / Jurkovich, Gregory; Pitt, Roger M.; Curreri, P. William; Granger, D. Neil.

In: Journal of Surgical Research, Vol. 44, No. 5, 01.01.1988, p. 514-521.

Research output: Contribution to journalArticle

Jurkovich, Gregory ; Pitt, Roger M. ; Curreri, P. William ; Granger, D. Neil. / Hypothermia prevents increased capillary permeability following ischemia-reperfusion injury. In: Journal of Surgical Research. 1988 ; Vol. 44, No. 5. pp. 514-521.
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