Hypervariable region IV of Salmonella gene fliC(d) encodes a dominant surface epitope and a stabilizing factor for functional flagella

Xiaosong He, M. Rivkina, B. A D Stocker, W. S. Robinson

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

To identify the major antigenic determinant of native Salmonella flagella of antigenic type d, we constructed a series of mutated fliC(d) genes with deletions and amino acid alterations in hypervariable region IV and in regions of putative epitopes as suggested by epitope mapping with synthetic octameric peptides (T.M. Joys and F. Schodel, Infect. Immun. 59:3330-3332, 1991). The expressed product of most of the mutant genes, with deletions of up to 92 amino acids in region IV, assembled into functional flagella and conferred motility on flagellin-deficient hosts. Serological analysis of these flagella with different anti-d antibodies revealed that the peptide sequence centered at amino acids 229 to 230 of flagellin was a dominant B- cell epitope at the surface of d flagella, because replacement of these two amino acids alone or together with their flanking sequence by a tripeptide specified by a linker sequence eliminated most reactivity with antisera against wild-type d flagella as tested by enzyme-linked immunosorbent assay or by Western immunoblot. Functional analysis of the mutated flagella genes with or without an insert suggested that amino acids 180 to 214 in the 5' part of hypervariable region IV (residues 181 to 307 of the total of 505) is important to the function of flagella. The hybrid proteins formed by insertion of peptide sequence pre-S1 12-47 of hepatitis B virus surface antigen into the deleted flagellins assembled into functional flagella, and antibody to the pre-S1 sequence was detected after immunization of mice with the hybrid protein. This suggests that such mutant flagellins containing heterologous epitopes have potential as vaccines.

Original languageEnglish (US)
Pages (from-to)2406-2414
Number of pages9
JournalJournal of Bacteriology
Volume176
Issue number8
StatePublished - 1994
Externally publishedYes

Fingerprint

Flagella
Salmonella
Epitopes
Flagellin
Genes
Amino Acids
Gene Deletion
Peptides
B-Lymphocyte Epitopes
Epitope Mapping
Insertional Mutagenesis
Hepatitis B Surface Antigens
Hepatitis B virus
Immune Sera
Immunization
Proteins
Vaccines
Western Blotting
Enzyme-Linked Immunosorbent Assay
Antibodies

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Immunology

Cite this

Hypervariable region IV of Salmonella gene fliC(d) encodes a dominant surface epitope and a stabilizing factor for functional flagella. / He, Xiaosong; Rivkina, M.; Stocker, B. A D; Robinson, W. S.

In: Journal of Bacteriology, Vol. 176, No. 8, 1994, p. 2406-2414.

Research output: Contribution to journalArticle

He, Xiaosong ; Rivkina, M. ; Stocker, B. A D ; Robinson, W. S. / Hypervariable region IV of Salmonella gene fliC(d) encodes a dominant surface epitope and a stabilizing factor for functional flagella. In: Journal of Bacteriology. 1994 ; Vol. 176, No. 8. pp. 2406-2414.
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