Hypervariable epitope constructs as a means of accounting for epitope variability

David E. Anderson, Arthur Malley, Eli Benjamini, Murray B. Gardner, Jose V Torres

Research output: Contribution to journalArticle

23 Scopus citations

Abstract

Epitope variability is one of the greatest obstacles to development of synthetic peptide vaccines. Based on a recently described hypervariable epitope (aa 414-434) on the envelope glycoprotein (gp130) to simian immunodeficiency virus (SIV mac 142), we have developed a novel approach to account for epitope variability. We have prepared, in a single synthesis, a cocktail of peptides, designated a hypervariable epitope construct (HEC), which collectively represent all the in vivo variability seen in an epitope. The HEC represents permutations of amino acid substitutions found in the epitope and has been able to induce antibodies with enhanced binding to native SIV and broad immunoreactivity to related epitope analogues.

Original languageEnglish (US)
Pages (from-to)736-740
Number of pages5
JournalVaccine
Volume12
Issue number8
DOIs
StatePublished - 1994

Keywords

  • epitope variability
  • Hypervariable epitope constructs
  • simian immunodeficiency virus

ASJC Scopus subject areas

  • Immunology
  • Microbiology
  • Virology
  • Infectious Diseases
  • Public Health, Environmental and Occupational Health
  • veterinary(all)

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