Abstract
Epitope variability is one of the greatest obstacles to development of synthetic peptide vaccines. Based on a recently described hypervariable epitope (aa 414-434) on the envelope glycoprotein (gp130) to simian immunodeficiency virus (SIV mac 142), we have developed a novel approach to account for epitope variability. We have prepared, in a single synthesis, a cocktail of peptides, designated a hypervariable epitope construct (HEC), which collectively represent all the in vivo variability seen in an epitope. The HEC represents permutations of amino acid substitutions found in the epitope and has been able to induce antibodies with enhanced binding to native SIV and broad immunoreactivity to related epitope analogues.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 736-740 |
| Number of pages | 5 |
| Journal | Vaccine |
| Volume | 12 |
| Issue number | 8 |
| DOIs | |
| State | Published - 1994 |
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Keywords
- epitope variability
- Hypervariable epitope constructs
- simian immunodeficiency virus
ASJC Scopus subject areas
- Immunology
- Microbiology
- Virology
- Infectious Diseases
- Public Health, Environmental and Occupational Health
- veterinary(all)
Cite this
Hypervariable epitope constructs as a means of accounting for epitope variability. / Anderson, David E.; Malley, Arthur; Benjamini, Eli; Gardner, Murray B.; Torres, Jose V.
In: Vaccine, Vol. 12, No. 8, 1994, p. 736-740.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Hypervariable epitope constructs as a means of accounting for epitope variability
AU - Anderson, David E.
AU - Malley, Arthur
AU - Benjamini, Eli
AU - Gardner, Murray B.
AU - Torres, Jose V
PY - 1994
Y1 - 1994
N2 - Epitope variability is one of the greatest obstacles to development of synthetic peptide vaccines. Based on a recently described hypervariable epitope (aa 414-434) on the envelope glycoprotein (gp130) to simian immunodeficiency virus (SIV mac 142), we have developed a novel approach to account for epitope variability. We have prepared, in a single synthesis, a cocktail of peptides, designated a hypervariable epitope construct (HEC), which collectively represent all the in vivo variability seen in an epitope. The HEC represents permutations of amino acid substitutions found in the epitope and has been able to induce antibodies with enhanced binding to native SIV and broad immunoreactivity to related epitope analogues.
AB - Epitope variability is one of the greatest obstacles to development of synthetic peptide vaccines. Based on a recently described hypervariable epitope (aa 414-434) on the envelope glycoprotein (gp130) to simian immunodeficiency virus (SIV mac 142), we have developed a novel approach to account for epitope variability. We have prepared, in a single synthesis, a cocktail of peptides, designated a hypervariable epitope construct (HEC), which collectively represent all the in vivo variability seen in an epitope. The HEC represents permutations of amino acid substitutions found in the epitope and has been able to induce antibodies with enhanced binding to native SIV and broad immunoreactivity to related epitope analogues.
KW - epitope variability
KW - Hypervariable epitope constructs
KW - simian immunodeficiency virus
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U2 - 10.1016/0264-410X(94)90225-9
DO - 10.1016/0264-410X(94)90225-9
M3 - Article
VL - 12
SP - 736
EP - 740
JO - Vaccine
JF - Vaccine
SN - 0264-410X
IS - 8
ER -