Hyperplastic polyps of the colon and rectum. An immunohistochemical study with monoclonal antibodies against blood groups antigens (Sialosyl-Lea, Leb, Le(x), Le(y), A, B, H)

H. S. Cooper; Carol S Marshall; F. Ruggerio; Z. Steplewski

Hyperplastic polyps of the colon and rectum. An immunohistochemical study with monoclonal antibodies against blood groups antigens (Sialosyl-Le^a, Le^b, Le(x), Le(y), A, B, H)

H. S. Cooper, Carol S Marshall, F. Ruggerio, Z. Steplewski

Research output: Contribution to journal › Article

Abstract

We studied 40 hyperplastic polyps (HP) immunohistochemically with monoclonal antibodies against 8 different blood group antigens (BGA) comparing their reactivity with normal control colon and colorectal adenocarcinomas. The 8 BGA studied were: Sialosyl-Le^a, Le^a, Le^b, Le(x), Le(y), A, B, and H. Sialosyl-Le^a, Le^a, Le(x), and Le(y) can be thought of as differentiation antigens. The former 2 BGA are expressed on mature (differentiated) epithelium while the latter 2 BGA are expressed by undifferentiated epithelium of the crypt base. A, B, H and Le^b are not expressed in the normal distal colon, however, they are extenively expressed on distal colorectal cancers and andenomas and can be considered onocofetal BGA. HP expressed Le^a, Le(x), Le(y) in the same compartment of the crypt as normal colon and extensively expressed Sialosyl-Le^a throughout the entire length of the crypt. This latter finding indicated maturation at a lower point in the crypt than in normals. All HP failed to express B and H BGA, while 6 of 40 expressed Le^b and 5 of 50 HP expressed A BGA. Of the 6 HP expressing Le^b BGA, 3 were from patients with synchronous or metachronous cancers and 2 from patients with mixed hyperplastic polyp-adenomas (HP/AD). Two of the HP expressing A BGA were from patients with HP/AD. The expression and distribution of these BGA in HP, especially the extensive expression of sialosyl-Le^a correlates with the known cell kinetics of HP. While nonneoplastic in nature, HP may occasionally express true oncofetal BGA. Similarly, the HP component of HP/AD may also express true onocofetal BGA. These data suggest that the lesions classified morphologically as HP may be antigenically heterogenous.

Original language	English (US)
Pages (from-to)	421-428
Number of pages	8
Journal	Laboratory Investigation
Volume	57
Issue number	4
State	Published - 1987
Externally published	Yes

Fingerprint

Blood Group Antigens

Polyps

Rectum

Colon

Monoclonal Antibodies

Epithelium

ABO Blood-Group System

Differentiation Antigens

Adenoma

Colorectal Neoplasms

Adenocarcinoma

ASJC Scopus subject areas

Pathology and Forensic Medicine

Cite this

Cooper, H. S., Marshall, C. S., Ruggerio, F., & Steplewski, Z. (1987). Hyperplastic polyps of the colon and rectum. An immunohistochemical study with monoclonal antibodies against blood groups antigens (Sialosyl-Le^a, Le^b, Le(x), Le(y), A, B, H). Laboratory Investigation, 57(4), 421-428.

Hyperplastic polyps of the colon and rectum. An immunohistochemical study with monoclonal antibodies against blood groups antigens (Sialosyl-Le^a, Le^b, Le(x), Le(y), A, B, H). / Cooper, H. S.; Marshall, Carol S; Ruggerio, F.; Steplewski, Z.

In: Laboratory Investigation, Vol. 57, No. 4, 1987, p. 421-428.

Research output: Contribution to journal › Article

Cooper, HS, Marshall, CS, Ruggerio, F & Steplewski, Z 1987, 'Hyperplastic polyps of the colon and rectum. An immunohistochemical study with monoclonal antibodies against blood groups antigens (Sialosyl-Le^a, Le^b, Le(x), Le(y), A, B, H)', Laboratory Investigation, vol. 57, no. 4, pp. 421-428.

Cooper HS, Marshall CS, Ruggerio F, Steplewski Z. Hyperplastic polyps of the colon and rectum. An immunohistochemical study with monoclonal antibodies against blood groups antigens (Sialosyl-Le^a, Le^b, Le(x), Le(y), A, B, H). Laboratory Investigation. 1987;57(4):421-428.

Cooper, H. S. ; Marshall, Carol S ; Ruggerio, F. ; Steplewski, Z. / Hyperplastic polyps of the colon and rectum. An immunohistochemical study with monoclonal antibodies against blood groups antigens (Sialosyl-Le^a, Le^b, Le(x), Le(y), A, B, H). In: Laboratory Investigation. 1987 ; Vol. 57, No. 4. pp. 421-428.

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