Hyperphagia as a mediator of renal disease initiation in obese Zucker rats

Frazier T. Stevenson, Carrie M. Wheeldon, Matthew D. Gades, Harry Van Goor, Judith S. Stern

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Objective: We sought to determine whether prevention of overeating would block the very earliest manifestations of renal injury in young obese Zucker rats (OZRs). Research Methods and Procedures: Three groups of rats were studied, obese (fa/fa) Zucker rats and lean (Fa/Fa). Zucker controls were allowed to feed ad libitum, whereas a group of obese (fa/fa) Zucker rats was pair-fed to the lean group. Urine albumin and serum lipids were studied weekly from 6 to 10 weeks of age. Renal pathology and renal glomerular gene expression were examined when the rats were killed at 10 weeks of age. Results: Obese rats fed ad libitum developed significant albuminuria by 6 weeks of age, increasing at each subsequent time-point. This increase was completely blocked by pair-feeding. Serum triglycerides were significantly increased in obese rats fed ad libitum vs. the other groups. Urine albumin correlated significantly with both body weight and serum triglyceride level. Renal histopathology was normal in all groups. Analysis of gene expression of glomerular proteins by reverse transcriptase-polymerase chain reaction revealed that pair-feeding attenuated the increased expression of glomerular desmin, fibronectin, and the 92-kDa collagenase that was seen in obese animals fed ad libitum. Discussion: Prevention of overeating in young OZR normalizes albuminuria and attenuates the pathogenic alterations in glomerular gene expression seen at the initiation of renal disease in obese animals allowed to feed ad libitum. This model may be relevant for studying the early end-organ effects of obesity.

Original languageEnglish (US)
Pages (from-to)492-499
Number of pages8
JournalObesity Research
Volume9
Issue number8
StatePublished - Aug 2001

Fingerprint

Zucker Rats
Hyperphagia
overeating
kidney diseases
Kidney
rats
Albuminuria
Gene Expression
kidneys
Triglycerides
Urine
Desmin
gene expression
albumins
Collagenases
Reverse Transcriptase Polymerase Chain Reaction
Serum
urine
Fibronectins
Serum Albumin

Keywords

  • Glomerular disease
  • Hyperlipidemia
  • Hyperphagia
  • Zucker rats

ASJC Scopus subject areas

  • Food Science
  • Endocrinology
  • Medicine (miscellaneous)
  • Endocrinology, Diabetes and Metabolism
  • Public Health, Environmental and Occupational Health

Cite this

Stevenson, F. T., Wheeldon, C. M., Gades, M. D., Van Goor, H., & Stern, J. S. (2001). Hyperphagia as a mediator of renal disease initiation in obese Zucker rats. Obesity Research, 9(8), 492-499.

Hyperphagia as a mediator of renal disease initiation in obese Zucker rats. / Stevenson, Frazier T.; Wheeldon, Carrie M.; Gades, Matthew D.; Van Goor, Harry; Stern, Judith S.

In: Obesity Research, Vol. 9, No. 8, 08.2001, p. 492-499.

Research output: Contribution to journalArticle

Stevenson, FT, Wheeldon, CM, Gades, MD, Van Goor, H & Stern, JS 2001, 'Hyperphagia as a mediator of renal disease initiation in obese Zucker rats', Obesity Research, vol. 9, no. 8, pp. 492-499.
Stevenson FT, Wheeldon CM, Gades MD, Van Goor H, Stern JS. Hyperphagia as a mediator of renal disease initiation in obese Zucker rats. Obesity Research. 2001 Aug;9(8):492-499.
Stevenson, Frazier T. ; Wheeldon, Carrie M. ; Gades, Matthew D. ; Van Goor, Harry ; Stern, Judith S. / Hyperphagia as a mediator of renal disease initiation in obese Zucker rats. In: Obesity Research. 2001 ; Vol. 9, No. 8. pp. 492-499.
@article{b03420a6414f4e67900dc394b0547526,
title = "Hyperphagia as a mediator of renal disease initiation in obese Zucker rats",
abstract = "Objective: We sought to determine whether prevention of overeating would block the very earliest manifestations of renal injury in young obese Zucker rats (OZRs). Research Methods and Procedures: Three groups of rats were studied, obese (fa/fa) Zucker rats and lean (Fa/Fa). Zucker controls were allowed to feed ad libitum, whereas a group of obese (fa/fa) Zucker rats was pair-fed to the lean group. Urine albumin and serum lipids were studied weekly from 6 to 10 weeks of age. Renal pathology and renal glomerular gene expression were examined when the rats were killed at 10 weeks of age. Results: Obese rats fed ad libitum developed significant albuminuria by 6 weeks of age, increasing at each subsequent time-point. This increase was completely blocked by pair-feeding. Serum triglycerides were significantly increased in obese rats fed ad libitum vs. the other groups. Urine albumin correlated significantly with both body weight and serum triglyceride level. Renal histopathology was normal in all groups. Analysis of gene expression of glomerular proteins by reverse transcriptase-polymerase chain reaction revealed that pair-feeding attenuated the increased expression of glomerular desmin, fibronectin, and the 92-kDa collagenase that was seen in obese animals fed ad libitum. Discussion: Prevention of overeating in young OZR normalizes albuminuria and attenuates the pathogenic alterations in glomerular gene expression seen at the initiation of renal disease in obese animals allowed to feed ad libitum. This model may be relevant for studying the early end-organ effects of obesity.",
keywords = "Glomerular disease, Hyperlipidemia, Hyperphagia, Zucker rats",
author = "Stevenson, {Frazier T.} and Wheeldon, {Carrie M.} and Gades, {Matthew D.} and {Van Goor}, Harry and Stern, {Judith S.}",
year = "2001",
month = "8",
language = "English (US)",
volume = "9",
pages = "492--499",
journal = "Obesity",
issn = "1930-7381",
publisher = "Wiley-Blackwell",
number = "8",

}

TY - JOUR

T1 - Hyperphagia as a mediator of renal disease initiation in obese Zucker rats

AU - Stevenson, Frazier T.

AU - Wheeldon, Carrie M.

AU - Gades, Matthew D.

AU - Van Goor, Harry

AU - Stern, Judith S.

PY - 2001/8

Y1 - 2001/8

N2 - Objective: We sought to determine whether prevention of overeating would block the very earliest manifestations of renal injury in young obese Zucker rats (OZRs). Research Methods and Procedures: Three groups of rats were studied, obese (fa/fa) Zucker rats and lean (Fa/Fa). Zucker controls were allowed to feed ad libitum, whereas a group of obese (fa/fa) Zucker rats was pair-fed to the lean group. Urine albumin and serum lipids were studied weekly from 6 to 10 weeks of age. Renal pathology and renal glomerular gene expression were examined when the rats were killed at 10 weeks of age. Results: Obese rats fed ad libitum developed significant albuminuria by 6 weeks of age, increasing at each subsequent time-point. This increase was completely blocked by pair-feeding. Serum triglycerides were significantly increased in obese rats fed ad libitum vs. the other groups. Urine albumin correlated significantly with both body weight and serum triglyceride level. Renal histopathology was normal in all groups. Analysis of gene expression of glomerular proteins by reverse transcriptase-polymerase chain reaction revealed that pair-feeding attenuated the increased expression of glomerular desmin, fibronectin, and the 92-kDa collagenase that was seen in obese animals fed ad libitum. Discussion: Prevention of overeating in young OZR normalizes albuminuria and attenuates the pathogenic alterations in glomerular gene expression seen at the initiation of renal disease in obese animals allowed to feed ad libitum. This model may be relevant for studying the early end-organ effects of obesity.

AB - Objective: We sought to determine whether prevention of overeating would block the very earliest manifestations of renal injury in young obese Zucker rats (OZRs). Research Methods and Procedures: Three groups of rats were studied, obese (fa/fa) Zucker rats and lean (Fa/Fa). Zucker controls were allowed to feed ad libitum, whereas a group of obese (fa/fa) Zucker rats was pair-fed to the lean group. Urine albumin and serum lipids were studied weekly from 6 to 10 weeks of age. Renal pathology and renal glomerular gene expression were examined when the rats were killed at 10 weeks of age. Results: Obese rats fed ad libitum developed significant albuminuria by 6 weeks of age, increasing at each subsequent time-point. This increase was completely blocked by pair-feeding. Serum triglycerides were significantly increased in obese rats fed ad libitum vs. the other groups. Urine albumin correlated significantly with both body weight and serum triglyceride level. Renal histopathology was normal in all groups. Analysis of gene expression of glomerular proteins by reverse transcriptase-polymerase chain reaction revealed that pair-feeding attenuated the increased expression of glomerular desmin, fibronectin, and the 92-kDa collagenase that was seen in obese animals fed ad libitum. Discussion: Prevention of overeating in young OZR normalizes albuminuria and attenuates the pathogenic alterations in glomerular gene expression seen at the initiation of renal disease in obese animals allowed to feed ad libitum. This model may be relevant for studying the early end-organ effects of obesity.

KW - Glomerular disease

KW - Hyperlipidemia

KW - Hyperphagia

KW - Zucker rats

UR - http://www.scopus.com/inward/record.url?scp=0035430881&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0035430881&partnerID=8YFLogxK

M3 - Article

C2 - 11500530

AN - SCOPUS:0035430881

VL - 9

SP - 492

EP - 499

JO - Obesity

JF - Obesity

SN - 1930-7381

IS - 8

ER -