Hyper-Crosslinked Carbohydrate Polymer for Repair of Critical-Sized Bone Defects

Plamena M. Koleva, James H. Keefer, Alexandria M. Ayala, Isabela Lorenzo, Christine E. Han, Kristen Pham, Stacy E. Ralston, Kee D. Kim, Charles C. Lee

Research output: Contribution to journalArticle


This study evaluated the safety and efficacy of a novel hyper-crosslinked carbohydrate polymer (HCCP) for the repair of critical-sized bone defects in comparison to two alternative treatments: autologous bone and poly(lactide-co-glycolide) with hyaluronic acid (PLGA/HA). Bilateral critical-sized defects were created in the lateral femoral condyles of skeletally mature New Zealand White rabbits, and they were subsequently implanted with HCCP, PLGA/HA, or autologous bone in a randomized manner. Clinical and behavioral observations were made daily, and radiological and histopathological evaluations were performed at 4, 10, and 16 weeks postimplantation. Defects implanted with HCCP showed progressive bone regeneration and bridging of the defect without adverse histological events. No signs of infection or inflammation associated with the implant material were observed in all animals that received HCCP implantation. A radiographic assessment performed at 16 weeks post-implantation showed significantly higher bone density and volume in defects implanted with HCCP compared to PLGA/HA. No statistically significant difference was observed in bone density and volume between HCCP and autologous bone. These findings demonstrate that HCCP is biocompatible, osteoconductive, and capable of promoting bone regeneration in vivo; therefore, it is suitable for both tissue engineering and the repair of critical-sized bone defects.

Original languageEnglish (US)
Pages (from-to)111-120
Number of pages10
JournalBioResearch Open Access
Issue number1
StatePublished - Jul 1 2019


  • bone graft substitute
  • critical-sized defect
  • hyper-crosslinked carbohydrate polymer
  • tissue engineering

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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