Abstract
Surface-enhanced Raman scattering (SERS) is a promising analytical technique for the detection and characterization of biological molecules and structures. The role of hydrophobic and hydrophilic surfaces in the self-assembly of protein-metallic nanoparticle structures for label-free protein detection is demonstrated. Aggregation is driven by both the hydrophobicity of the surface as well as the charge of the proteins. The best conditions for obtaining a reproducible SERS signal that allows for sensitive, label-free protein detection are provided by the use of hydrophobic surfaces and 16 × 1011 NPs per mL. A detection limit of approximately 0.5 μg mL-1 is achieved regardless of the proteins' charge properties and size. The developed method is simple and can be used for reproducible and sensitive detection and characterization of a wide variety of biological molecules and various structures with different sizes and charge status.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 2906-2913 |
| Number of pages | 8 |
| Journal | Analyst |
| Volume | 138 |
| Issue number | 10 |
| DOIs | |
| State | Published - May 21 2013 |
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ASJC Scopus subject areas
- Analytical Chemistry
- Spectroscopy
- Electrochemistry
- Biochemistry
- Environmental Chemistry
- Medicine(all)
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Hydrophobicity-driven self-assembly of protein and silver nanoparticles for protein detection using surface-enhanced Raman scattering. / Kahraman, Mehmet; Balz, Ben N.; Wachsmann-Hogiu, Sebastian.
In: Analyst, Vol. 138, No. 10, 21.05.2013, p. 2906-2913.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Hydrophobicity-driven self-assembly of protein and silver nanoparticles for protein detection using surface-enhanced Raman scattering
AU - Kahraman, Mehmet
AU - Balz, Ben N.
AU - Wachsmann-Hogiu, Sebastian
PY - 2013/5/21
Y1 - 2013/5/21
N2 - Surface-enhanced Raman scattering (SERS) is a promising analytical technique for the detection and characterization of biological molecules and structures. The role of hydrophobic and hydrophilic surfaces in the self-assembly of protein-metallic nanoparticle structures for label-free protein detection is demonstrated. Aggregation is driven by both the hydrophobicity of the surface as well as the charge of the proteins. The best conditions for obtaining a reproducible SERS signal that allows for sensitive, label-free protein detection are provided by the use of hydrophobic surfaces and 16 × 1011 NPs per mL. A detection limit of approximately 0.5 μg mL-1 is achieved regardless of the proteins' charge properties and size. The developed method is simple and can be used for reproducible and sensitive detection and characterization of a wide variety of biological molecules and various structures with different sizes and charge status.
AB - Surface-enhanced Raman scattering (SERS) is a promising analytical technique for the detection and characterization of biological molecules and structures. The role of hydrophobic and hydrophilic surfaces in the self-assembly of protein-metallic nanoparticle structures for label-free protein detection is demonstrated. Aggregation is driven by both the hydrophobicity of the surface as well as the charge of the proteins. The best conditions for obtaining a reproducible SERS signal that allows for sensitive, label-free protein detection are provided by the use of hydrophobic surfaces and 16 × 1011 NPs per mL. A detection limit of approximately 0.5 μg mL-1 is achieved regardless of the proteins' charge properties and size. The developed method is simple and can be used for reproducible and sensitive detection and characterization of a wide variety of biological molecules and various structures with different sizes and charge status.
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UR - http://www.scopus.com/inward/citedby.url?scp=84876722294&partnerID=8YFLogxK
U2 - 10.1039/c3an00025g
DO - 10.1039/c3an00025g
M3 - Article
VL - 138
SP - 2906
EP - 2913
JO - The Analyst
JF - The Analyst
SN - 0003-2654
IS - 10
ER -