Hydrogen production by a hyperthermophilic membrane-bound hydrogenase in water-soluble nanolipoprotein particles

Sarah E. Baker, Robert C. Hopkins, Craig D. Blanchette, Vicki L. Walsworth, Rhoda Sumbad, Nicholas O Fischer, Edward A. Kuhn, Matthew A Coleman, Brett A. Chromy, Sonia E. Létant, Paul D. Hoeprich, Michael W W Adams, Paul Henderson

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

(Chemical Equation Presented) Hydrogenases constitute a promising class of enzymes for ex vivo hydrogen production. Implementation of such applications is currently hindered by oxygen sensitivity and, in the case of membrane-bound hydrogenases (MBHs), poor water solubility. Nanolipoprotein particles (NLPs) formed from apolipoproteins and phospholipids offer a novel means of incorporating MBHs into a well-defined water-soluble matrix that maintains the enzymatic activity and is amenable to incorporation into more complex architectures. We report the synthesis, hydrogen-evolving activity, and physical characterization of the first MBH-NLP assembly. This may ultimately lead to the development of biomimetic hydrogen-production devices.

Original languageEnglish (US)
Pages (from-to)7508-7509
Number of pages2
JournalJournal of the American Chemical Society
Volume131
Issue number22
DOIs
StatePublished - Jun 10 2009

Fingerprint

Hydrogenase
Hydrogen production
Hydrogen
Membranes
Water
Phospholipids
Biomimetics
Apolipoproteins
Enzymes
Solubility
Oxygen
Equipment and Supplies

ASJC Scopus subject areas

  • Chemistry(all)
  • Catalysis
  • Biochemistry
  • Colloid and Surface Chemistry

Cite this

Hydrogen production by a hyperthermophilic membrane-bound hydrogenase in water-soluble nanolipoprotein particles. / Baker, Sarah E.; Hopkins, Robert C.; Blanchette, Craig D.; Walsworth, Vicki L.; Sumbad, Rhoda; Fischer, Nicholas O; Kuhn, Edward A.; Coleman, Matthew A; Chromy, Brett A.; Létant, Sonia E.; Hoeprich, Paul D.; Adams, Michael W W; Henderson, Paul.

In: Journal of the American Chemical Society, Vol. 131, No. 22, 10.06.2009, p. 7508-7509.

Research output: Contribution to journalArticle

Baker, SE, Hopkins, RC, Blanchette, CD, Walsworth, VL, Sumbad, R, Fischer, NO, Kuhn, EA, Coleman, MA, Chromy, BA, Létant, SE, Hoeprich, PD, Adams, MWW & Henderson, P 2009, 'Hydrogen production by a hyperthermophilic membrane-bound hydrogenase in water-soluble nanolipoprotein particles', Journal of the American Chemical Society, vol. 131, no. 22, pp. 7508-7509. https://doi.org/10.1021/ja809251f
Baker, Sarah E. ; Hopkins, Robert C. ; Blanchette, Craig D. ; Walsworth, Vicki L. ; Sumbad, Rhoda ; Fischer, Nicholas O ; Kuhn, Edward A. ; Coleman, Matthew A ; Chromy, Brett A. ; Létant, Sonia E. ; Hoeprich, Paul D. ; Adams, Michael W W ; Henderson, Paul. / Hydrogen production by a hyperthermophilic membrane-bound hydrogenase in water-soluble nanolipoprotein particles. In: Journal of the American Chemical Society. 2009 ; Vol. 131, No. 22. pp. 7508-7509.
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