Hydrogen peroxide production by red blood cells

Cecilia R Giulivi, Paul Hochstein, Kelvin J A Davies

Research output: Contribution to journalArticle

103 Scopus citations

Abstract

Red blood cells are frequently employed in studies of oxidative stress. Technical difficulties have previously prevented the measurement of H2O2 production by red blood cells, except during exposure to certain drugs or toxicants. We now show that a combination of glutathione depletion and 3-amino-1,2,4-triazole (aminotriazole) treatment can be used to measure the endogenous generation of H2O2 by red blood cells. In our studies, aminotriazole was used as an H2O2 dependent (irreversible) catalase inhibitor, and catalase inhibition was used as an indirect measure of H2O2 production. Our results indicate that H2O2 is generated at a rate of 1.36 ± 0.2 μM/h (3.9 ± 0.6 nmol·h-1·gHb-1), and that the steady-state red blood cell concentration of H2O2 is approximately 2 × 10-10M. Kinetic comparisons of H2O2 production and oxyhemoglobin autooxidation (which generates O2 .- that dismutes to H2O2) indicate that the latter is probably the main source of H2O2 in red blood cells.

Original languageEnglish (US)
Pages (from-to)123-129
Number of pages7
JournalFree Radical Biology and Medicine
Volume16
Issue number1
DOIs
StatePublished - 1994
Externally publishedYes

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Keywords

  • Autoxidation
  • Free radicals
  • Hydrogen peroxide
  • Oxyhemoglobin
  • Red blood cells
  • Superoxide anion

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Medicine(all)
  • Toxicology

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