Hydrogel matrix to support stem cell survival after brain transplantation in stroke

Jin Zhong, Albert Chan, Leeron Morad, Harley I. Kornblum, Fan Guoping Fan, S. Thomas Carmichael

Research output: Contribution to journalArticle

118 Citations (Scopus)

Abstract

Stroke is a leading cause of adult disability. Stem/progenitor cell transplantation improves recovery after stroke in rodent models. These studies have 2 main limitations to clinical translation. First, most of the cells in stem/progenitor transplants die after brain transplantation. Second, intraparenchymal approaches target transplants to normal brain adjacent to the stroke, which is the site of the most extensive natural recovery in humans. Transplantation may damage this tissue. The stroke cavity provides an ideal target for transplantation because it is a compartmentalized region of necrosis, can accept a high volume transplant without tissue damage, and lies directly adjacent to the most plastic brain area in stroke. However, direct transplantation into the stroke cavity has caused massive death in the transplant. To overcome these limitations, the authors tested stem/progenitor transplants within a specific biopolymer hydrogel matrix to create a favorable environment for transplantation into the infarct cavity after stroke, and they tested this in comparison to stem cell injection without hydrogel support. A biopolymer hydrogel composed of cross-linked hyaluronan and heparin sulfate significantly promoted the survival of 2 different neural progenitor cell lines in vitro in conditions of stress and in vivo into the infarct cavity. Quantitative analysis of the transplant and surrounding tissue indicates diminished inflammatory infiltration of the graft with the hydrogel transplant. This result indicates that altering the local environment in stem cell transplantation enhances survival and diminishes cell stress. Stem cell transplantation into the infarct cavity within a pro-survival hydrogel matrix may provide a translational therapy for stroke recovery.

Original languageEnglish (US)
Pages (from-to)636-644
Number of pages9
JournalNeurorehabilitation and Neural Repair
Volume24
Issue number7
DOIs
StatePublished - Sep 2010

Fingerprint

Hydrogel
Cell Survival
Stem Cells
Transplantation
Stroke
Transplants
Brain
Stem Cell Transplantation
Biopolymers
Hyaluronic Acid
Plastics
Sulfates
Heparin
Rodentia
Necrosis
Cell Line
Injections
Survival

Keywords

  • bioengineering
  • gliosis
  • infarct cavity
  • inflammation
  • neovascularization
  • neural repair
  • stem cell

ASJC Scopus subject areas

  • Clinical Neurology
  • Rehabilitation
  • Neurology

Cite this

Zhong, J., Chan, A., Morad, L., Kornblum, H. I., Guoping Fan, F., & Carmichael, S. T. (2010). Hydrogel matrix to support stem cell survival after brain transplantation in stroke. Neurorehabilitation and Neural Repair, 24(7), 636-644. https://doi.org/10.1177/1545968310361958

Hydrogel matrix to support stem cell survival after brain transplantation in stroke. / Zhong, Jin; Chan, Albert; Morad, Leeron; Kornblum, Harley I.; Guoping Fan, Fan; Carmichael, S. Thomas.

In: Neurorehabilitation and Neural Repair, Vol. 24, No. 7, 09.2010, p. 636-644.

Research output: Contribution to journalArticle

Zhong, J, Chan, A, Morad, L, Kornblum, HI, Guoping Fan, F & Carmichael, ST 2010, 'Hydrogel matrix to support stem cell survival after brain transplantation in stroke', Neurorehabilitation and Neural Repair, vol. 24, no. 7, pp. 636-644. https://doi.org/10.1177/1545968310361958
Zhong, Jin ; Chan, Albert ; Morad, Leeron ; Kornblum, Harley I. ; Guoping Fan, Fan ; Carmichael, S. Thomas. / Hydrogel matrix to support stem cell survival after brain transplantation in stroke. In: Neurorehabilitation and Neural Repair. 2010 ; Vol. 24, No. 7. pp. 636-644.
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