Hybridization properties of oligodeoxynucleotide pairs bridged by polyarginine peptides

Ziping Wei, Ching Hsuan Tung, Tianmin Zhu, Walter A. Dickerhof, Kenneth J. Breslauer, Denise E. Georgopoulos, Michael J Leibowitz, Stanley Stein

Research output: Contribution to journalArticle

28 Scopus citations

Abstract

The hybridization properties of a series of probes, based on two 9mer oligodeoxynucleotides (designated as I and II) having an appended oligoarginine chain (R(n)) to produce peptide-oligonucleotide conjugates or peptide-bridged oligonucleotide pairs (e.g. R(n)-I or II-R(n)-I), were investigated. For the double-linked probes, we found that the peptide bridge induces the two 9mers to bind complementary single-stranded DNA or RNA targets with substantially enhanced thermal stability. The resulting hybrid with complementary DNA was found to assume a 1:1 complex in the B conformation as judged by UV mixing curves and CD spectroscopy. Complexes of single or double-linked probes with complementary RNA exhibited sensitivity to RNase H digestion. The influence of the identity and chirality of the repeating unit in the bridge, the length of the bridge, the gap size and the salt concentration on the hybridization properties of this new class of oligonucleotide probes was also studied. Our data reveal that these compounds exhibit properties that should prove useful in the development of antisense strategies.

Original languageEnglish (US)
Pages (from-to)655-661
Number of pages7
JournalNucleic Acids Research
Volume24
Issue number4
DOIs
StatePublished - 1996
Externally publishedYes

ASJC Scopus subject areas

  • Genetics

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    Wei, Z., Tung, C. H., Zhu, T., Dickerhof, W. A., Breslauer, K. J., Georgopoulos, D. E., Leibowitz, M. J., & Stein, S. (1996). Hybridization properties of oligodeoxynucleotide pairs bridged by polyarginine peptides. Nucleic Acids Research, 24(4), 655-661. https://doi.org/10.1093/nar/24.4.655