Hybrid peptide dendrimers for imaging of chemokine receptor 4 (CXCR4) expression

Joeri Kuil, Tessa Buckle, Joppe Oldenburg, Hushan Yuan, Alexander D Borowsky, Lee Josephson, Fijs W B Van Leeuwen

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

The chemokine receptor 4 (CXCR4), which is overexpressed in many types of cancer, is an emerging target in the field of molecular imaging and therapeutics. The CXCR4 binding of several peptides, including the cyclic Ac-TZ14011, has already been validated. In this study mono-, di- and tetrameric Ac-TZ14011-containing dendrimers were prepared and functionalized with a multimodal (hybrid) label, consisting of a Cy5.5-like fluorophore and a DTPA chelate. Confocal microscopy revealed that all three dendrimers were membrane bound at 4 °C, consistent with CXCR4 binding in vitro. The unlabeled dimer and tetramer had a somewhat lower affinity for CXCR4 than the unlabeled monomer. However, when labeled with the multimodal label the CXCR4 affinity of the dimer and tetramer was considerably higher compared to that of the labeled monomer. On top of that, biodistribution studies revealed that the additional peptides in the dimer and tetramer reduced nonspecific muscle uptake. Thus, multimerization of the cyclic Ac-TZ14011 peptide reduces the negative influence of the multimodal label on the receptor affinity and the biodistribution.

Original languageEnglish (US)
Pages (from-to)2444-2453
Number of pages10
JournalMolecular Pharmaceutics
Volume8
Issue number6
DOIs
StatePublished - Dec 5 2011

Fingerprint

Dendrimers
Chemokine Receptors
Affinity Labels
Cyclic Peptides
Pentetic Acid
Peptides
Molecular Imaging
Confocal Microscopy
Muscles
Membranes
Neoplasms
Therapeutics
In Vitro Techniques
CY5.5 cyanine dye

Keywords

  • CXCR4
  • dendrimers
  • fluorescence
  • multimodal hybrid imaging
  • peptides
  • SPECT/CT

ASJC Scopus subject areas

  • Pharmaceutical Science
  • Molecular Medicine
  • Drug Discovery

Cite this

Kuil, J., Buckle, T., Oldenburg, J., Yuan, H., Borowsky, A. D., Josephson, L., & Van Leeuwen, F. W. B. (2011). Hybrid peptide dendrimers for imaging of chemokine receptor 4 (CXCR4) expression. Molecular Pharmaceutics, 8(6), 2444-2453. https://doi.org/10.1021/mp200401p

Hybrid peptide dendrimers for imaging of chemokine receptor 4 (CXCR4) expression. / Kuil, Joeri; Buckle, Tessa; Oldenburg, Joppe; Yuan, Hushan; Borowsky, Alexander D; Josephson, Lee; Van Leeuwen, Fijs W B.

In: Molecular Pharmaceutics, Vol. 8, No. 6, 05.12.2011, p. 2444-2453.

Research output: Contribution to journalArticle

Kuil, J, Buckle, T, Oldenburg, J, Yuan, H, Borowsky, AD, Josephson, L & Van Leeuwen, FWB 2011, 'Hybrid peptide dendrimers for imaging of chemokine receptor 4 (CXCR4) expression', Molecular Pharmaceutics, vol. 8, no. 6, pp. 2444-2453. https://doi.org/10.1021/mp200401p
Kuil, Joeri ; Buckle, Tessa ; Oldenburg, Joppe ; Yuan, Hushan ; Borowsky, Alexander D ; Josephson, Lee ; Van Leeuwen, Fijs W B. / Hybrid peptide dendrimers for imaging of chemokine receptor 4 (CXCR4) expression. In: Molecular Pharmaceutics. 2011 ; Vol. 8, No. 6. pp. 2444-2453.
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