Hyaluronic acid enhances the zona pellucida-induced acrosome reaction of macaque sperm

Catherine A. VandeVoort, Gary N. Cherr, James W. Overstreet

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

Hyaluronic acid (HA) not only surrounds the zona pellucida as part of the cumulus matrix but also is present throughout the zona pellucida and the perivitelline space of many mammalian oocytes. However, most in vitro techniques to study sperm-oocyte interaction eliminate HA from the oocyte through enzymatic digestion and/or do not expose sperm to HA prior to zona pellucida binding. This study explores the effect of preincubation of sperm or oocytes with HA on sperm-zona pellucida binding and subsequent acrosome reaction of bound sperm. Cynomolgus macaque semen was washed, incubated, chemically capacitated with dibutryl cyclic adenosine monophosphate (dbcAMP) and caffeine, and sperm-zona pellucida binding assays were performed. In one experiment, sperm were pretreated with HA (100 μg/ml) during the last 10 minutes of the 30-minute period for chemical capacitation. In another experiment, only the oocytes were preincubated in the media containing HA. In the third experiment, gametes were exposed to HA for 10 minutes after sperm had been allowed to bind to zonae pellucida. The preincubation of either sperm or zonae pellucida with HA enhanced the percentage of bound sperm that were acrosome reacted. However, HA did not affect the number of sperm bound to zonae pellucida. When sperm already bound to the zona pellucida were exposed to HA, there was no increase in the percentage of bound acrosome- reacted sperm. The HA enhancement of the acrosome reaction of sperm bound to the zona pellucida shown in this study required less than 1 minute of sperm exposure to HA-treated zonae during the zona binding process. However, this enhancement was observed only if the HA exposure preceded sperm-zona binding. This result suggests that HA is interacting with the sperm surface, possibly via a receptor, at the time of initiation of the acrosome reaction. It is unlikely that the effects noted in the current experiments were the result of tactility retention or improvement because the full enhancement of the acrosome reaction was observed when only the oocytes were pretreated with HA.

Original languageEnglish (US)
Pages (from-to)1-5
Number of pages5
JournalJournal of Andrology
Volume18
Issue number1
StatePublished - 1997

Fingerprint

Acrosome Reaction
Zona Pellucida
Macaca
Hyaluronic Acid
Spermatozoa
Oocytes
Herpes Zoster
Acrosome
Sperm-Ovum Interactions
Sperm Count
Caffeine
Semen

Keywords

  • capacitation
  • fertilization
  • Macaca fascicularis
  • semen

ASJC Scopus subject areas

  • Endocrinology

Cite this

VandeVoort, C. A., Cherr, G. N., & Overstreet, J. W. (1997). Hyaluronic acid enhances the zona pellucida-induced acrosome reaction of macaque sperm. Journal of Andrology, 18(1), 1-5.

Hyaluronic acid enhances the zona pellucida-induced acrosome reaction of macaque sperm. / VandeVoort, Catherine A.; Cherr, Gary N.; Overstreet, James W.

In: Journal of Andrology, Vol. 18, No. 1, 1997, p. 1-5.

Research output: Contribution to journalArticle

VandeVoort, CA, Cherr, GN & Overstreet, JW 1997, 'Hyaluronic acid enhances the zona pellucida-induced acrosome reaction of macaque sperm', Journal of Andrology, vol. 18, no. 1, pp. 1-5.
VandeVoort, Catherine A. ; Cherr, Gary N. ; Overstreet, James W. / Hyaluronic acid enhances the zona pellucida-induced acrosome reaction of macaque sperm. In: Journal of Andrology. 1997 ; Vol. 18, No. 1. pp. 1-5.
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