Humoral response of cattle to aerosolized Micropolyspora faeni.

L. J. Gershwin, C. L. Olsen

Research output: Contribution to journalArticle

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Abstract

Calves (7) were exposed to antigens of Micropolyspora faeni by the aerosol route for 9 weeks. The humoral immune response of calves to M faeni antigens was studied; immunoglobulins (Ig) E, G1, G2, A, and M were measured weekly in serum and nasal secretions by enzyme-linked immunosorbent assay (ELISA). Intradermal injection of antigen was performed during the 6th and 9th weeks; responses were evaluated at 30 minutes, 6 to 8 hours, 24, and 48 hours after injection. Total IgE levels in serum and nasal secretions, evaluated weekly, did not show any elevation. Micropolyspora faeni-specific IgE, IgA, IgG1, and IgG2, but not IgM, were produced by calves exposed to the antigen by the aerosol route; individual variability in magnitude of the response was marked. Thirty-minute skin tests were positive for cytotropic antibody in 2 of 3 aerosol-exposed calves by the 9th week, but delayed-type reactivity was not present. The ELISA test results were compared with those from sera of saline solution aerosol-exposed calves and from a parenterally immunized calf. Comparison of isotype-specific ELISA results obtained from M faeni aerosol-exposed calves with ELISA results from calves exposed to aerosolized ovalbumin according to a similar procedure indicated inherent problems in evaluating immune responses to environmental antigens. Aerosolized M faeni elicited a substantial antibody response. In particular, it is noteworthy that antigen-specific IgE responses were detected.

Original languageEnglish (US)
Pages (from-to)2135-2142
Number of pages8
JournalAmerican Journal of Veterinary Research
Volume45
Issue number10
StatePublished - Oct 1984

Fingerprint

Saccharopolyspora rectivirgula
Nocardia
humoral immunity
Aerosols
calves
Antigens
aerosols
Immunoglobulin E
cattle
antigens
Enzyme-Linked Immunosorbent Assay
enzyme-linked immunosorbent assay
Nose
Immunoglobulin G
Serum
Intradermal Injections
Ovalbumin
secretion
Humoral Immunity
injection

ASJC Scopus subject areas

  • veterinary(all)

Cite this

Humoral response of cattle to aerosolized Micropolyspora faeni. / Gershwin, L. J.; Olsen, C. L.

In: American Journal of Veterinary Research, Vol. 45, No. 10, 10.1984, p. 2135-2142.

Research output: Contribution to journalArticle

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