Humoral response in neonatal calves following immunization with Escherichia coli (strain J5)

The effects of adjuvant, age and colostral passive interference

Jeff W. Tyler, James S Cullor, Mark Thurmond, Vicki L. Douglas, J. D. Dellinger

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Serologic responses in 61 calves 3 to 34 days of age following immunization with bacterins containing a heat-killed rough mutant, Escherichia coli 0111:B4 (strain J5) were determined by an enzyme-linked immunosorbent assay specific for the IgG isotype. Administration of either heat-killed bacteria or oil-based adjuvants alone failed to enhance serologic recognition of common core antigens when comparing to nonvaccinate controls. Increased titers were uniquely and specifically limited to calves receiving the antigen in an oil emulsion. In a second experiment, age and initial, passively acquired titer recognizing the vaccinal antigen were not found to have any effect on the magnitude of the humoral response of 57 calves following immunization.

Original languageEnglish (US)
Pages (from-to)333-344
Number of pages12
JournalVeterinary Immunology and Immunopathology
Volume23
Issue number3-4
DOIs
StatePublished - 1989

Fingerprint

crossover interference
humoral immunity
adjuvants
Immunization
immunization
calves
Escherichia coli
antigens
Antigens
Oils
Hot Temperature
Bacterial Vaccines
heat
oils
Emulsions
emulsions
Immunoglobulin G
Enzyme-Linked Immunosorbent Assay
enzyme-linked immunosorbent assay
Bacteria

ASJC Scopus subject areas

  • Animal Science and Zoology
  • Immunology
  • veterinary(all)

Cite this

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abstract = "Serologic responses in 61 calves 3 to 34 days of age following immunization with bacterins containing a heat-killed rough mutant, Escherichia coli 0111:B4 (strain J5) were determined by an enzyme-linked immunosorbent assay specific for the IgG isotype. Administration of either heat-killed bacteria or oil-based adjuvants alone failed to enhance serologic recognition of common core antigens when comparing to nonvaccinate controls. Increased titers were uniquely and specifically limited to calves receiving the antigen in an oil emulsion. In a second experiment, age and initial, passively acquired titer recognizing the vaccinal antigen were not found to have any effect on the magnitude of the humoral response of 57 calves following immunization.",
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