Humoral immune responses to phocine herpesvirus-1 in Pacific harbor seals (Phoca vitulina richardsii) during an outbreak of clinical disease

Donald P. King, Andrew R. Lie, Tracey Goldstein, Brian M. Aldridge, Frances M D Gulland, Martin Haulena, Mark A. Adkison, Linda J Lowenstine, Jeffrey L Stott

Research output: Contribution to journalArticle

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Abstract

Infection with phocine herpesvirus type-1 (PHV-1) has been associated with morbidity and high mortality in neonatal harbor seals (Phoca vitulina). A PHV-1 specific indirect enzyme linked immunosorbent assay (ELISA) was developed to sequentially measure the serological status of 106 harbor seal neonates admitted to a Pacific coast rehabilitation center (total number of sera tested was 371). Early in the season (February-April), the majority of pups had low serum levels of PHV-1 specific antibody. A dramatic increase in PHV-1 specific antibody, involving the majority of hospitalized pups, was observed during a 4-week period in May. This coincided with a high incidence of PHV-1 associated adrenal lesions and mortality. Although there was overall agreement between the timing of seroconversion to PHV-1 and histological evidence of PHV-1 infection, 82.4% of individual pups with adrenalitis had no evidence of a humoral response to PHV-1 at the time of their death. This suggests either a rapid disease course, or an inability to develop a humoral response in some neonatal seals.

Original languageEnglish (US)
Pages (from-to)1-8
Number of pages8
JournalVeterinary Microbiology
Volume80
Issue number1
DOIs
StatePublished - May 3 2001

Fingerprint

Phoca
Phoca vitulina
Herpesviridae
Humoral Immunity
humoral immunity
pups
Disease Outbreaks
neonatal mortality
antibodies
seroconversion
disease course
lesions (animal)
seals
infection
morbidity
neonates
enzyme-linked immunosorbent assay
death
coasts
incidence

Keywords

  • ELISA
  • Immunity
  • Phocine herpesvirus
  • Seal
  • Serology

ASJC Scopus subject areas

  • Animal Science and Zoology
  • Microbiology
  • veterinary(all)

Cite this

Humoral immune responses to phocine herpesvirus-1 in Pacific harbor seals (Phoca vitulina richardsii) during an outbreak of clinical disease. / King, Donald P.; Lie, Andrew R.; Goldstein, Tracey; Aldridge, Brian M.; Gulland, Frances M D; Haulena, Martin; Adkison, Mark A.; Lowenstine, Linda J; Stott, Jeffrey L.

In: Veterinary Microbiology, Vol. 80, No. 1, 03.05.2001, p. 1-8.

Research output: Contribution to journalArticle

King, Donald P. ; Lie, Andrew R. ; Goldstein, Tracey ; Aldridge, Brian M. ; Gulland, Frances M D ; Haulena, Martin ; Adkison, Mark A. ; Lowenstine, Linda J ; Stott, Jeffrey L. / Humoral immune responses to phocine herpesvirus-1 in Pacific harbor seals (Phoca vitulina richardsii) during an outbreak of clinical disease. In: Veterinary Microbiology. 2001 ; Vol. 80, No. 1. pp. 1-8.
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