Humoral immune response to p53 correlates with clinical course in colorectal cancer patients during adjuvant chemotherapy

Mirna Lechpammer, Josip Lukač, Stanislav Lechpammer, Dujo Kovačević, Massimo Loda, Zvonko Kusić

Research output: Contribution to journalArticle

44 Scopus citations

Abstract

Background and aims: Overexpression of p53 protein in malignancies induces an immune response in some cancer patients. We investigated whether production of serum antibodies against p53 (p53-Ab) is associated with pathohistological parameters of colorectal carcinoma and whether p53-Ab can serve as a tumor marker during cancer treatment. Patients and methods: Serum samples from 220 colorectal cancer patients during surgery and adjuvant chemotherapy and 42 healthy controls were tested for the presence of p53-Ab by ELISA. Expression of p53 protein in tumors was determined using mouse anti-human p53-Ab. Results: Serum p53-Ab were detected in 18% of patients while all controls were negative. A strong correlation between p53-Ab production and p53 protein expression was observed: 70% of p53-Ab positive cases had tumors positive for p53 vs. 52% of p53-Ab negative cases. There was also a significant predominance of p53-Ab positive cases in Dukes' stages B and C over stage A. Although surgery alone reduced p53-Ab levels, decreases in p53-Ab titer became significant midterm through chemotherapy compared to both pre- and postoperative values and remained decreased until the completion of treatment. Conclusion: The presence of p53-Ab in sera of patients with colorectal cancer indicates tumors in more advanced histopathologic stages (Dukes' B, C). Due to low sensitivity (18%) p53-Ab are not recommendable as a preoperative marker for colorectal cancer. However, due to high specificity (100%), their monitoring after surgery and adjuvant chemotherapy has potential for early diagnosis of tumor relapse in p53-Ab positive cases.

Original languageEnglish (US)
Pages (from-to)114-120
Number of pages7
JournalInternational Journal of Colorectal Disease
Volume19
Issue number2
DOIs
StatePublished - Mar 2004
Externally publishedYes

Keywords

  • Cancer treatment follow-up
  • Colorectal cancer
  • Serum p53 antibodies
  • Tumor suppressor gene p53

ASJC Scopus subject areas

  • Gastroenterology

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