Humoral immune response of calves to bluetongue virus infection.

Nigel J Maclachlan, H. W. Heidner, F. J. Fuller

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Humoral immune responses of 7 calves to bluetongue virus (BTV) infection were evaluated by plaque-reduction assay and immunoblotting. Most readily interpretable results were obtained with the immunoblot assay when colostrum-deprived calves were used, and sera were reacted with proteins in partially purified extracts of BTV. Viremia persisted in calves for 35 to 56 days, and BTV coexisted in blood for several weeks with virus-specific neutralizing antibody. Calves developed antibody to virus protein 2, the major determinant of virus neutralization, at 14 to 28 days after inoculation; this time interval also coincided with the appearance of neutralizing antibody in serum. Virus clearance in BTV-infected calves did not coincide with humoral immune responses to protein 2 or other virion proteins.

Original languageEnglish (US)
Pages (from-to)1031-1035
Number of pages5
JournalAmerican Journal of Veterinary Research
Volume48
Issue number7
StatePublished - Jul 1987
Externally publishedYes

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Bluetongue virus
Virus Diseases
Humoral Immunity
humoral immunity
calves
Viruses
Neutralizing Antibodies
infection
blood serum
Proteins
neutralizing antibodies
viruses
proteins
Colostrum
Viremia
Serum
Immunoblotting
Virion
viremia
neutralization tests

ASJC Scopus subject areas

  • veterinary(all)

Cite this

Humoral immune response of calves to bluetongue virus infection. / Maclachlan, Nigel J; Heidner, H. W.; Fuller, F. J.

In: American Journal of Veterinary Research, Vol. 48, No. 7, 07.1987, p. 1031-1035.

Research output: Contribution to journalArticle

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