Humoral immune response associated with Lyme borreliosis in nonhuman primates: Analysis by immunoblotting and enzyme-linked immunosorbent assay with sonicates or recombinant proteins

A. R. Pachner, D. Dail, L. Li, L. Gurey, S. Feng, Emir Hodzic, Stephen W Barthold

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

The immune response to Borrelia burgdorferi, the causative agent of Lyme disease, is complex. We studied the immunoglobulin M (IgM) and IgG antibody response to N40Br, a sensu stricto strain, in the rhesus macaque-(nonhuman primate [NHP]) model of infection to identify the spirochetal protein targets of specific antibody. Antigens used in enzyme-linked immunosorbent assays were whole-cell sonicates of the spirochete and recombinant proteins of B. burgdorferi. Immunoblotting with a commercially available strip and subsequent quantitative densitometry of the bands were also used. Sera from four different groups of NHPs were used: immunocompetent, transiently immunosuppressed, extended immunosuppressed, and uninfected. In immunocompetent and transiently immunosuppressed NHPs, there was a strong IgM and IgG response. Major proteins for the early IgM response were P39 and P41 and recombinant BmpA and OspC. Major proteins for the later IgG response were P39, P41, P18, P60, P66, and recombinant BmpA and DbpA. There was no significant response in the NHPs to recombinant OspA or to Arp, a 37-kDa protein that elicits an antibody response during infection in mice. Most antibody responses, except for that to DbpA, were markedly diminished by prolonged dexamethasone treatment. This study supports the hypothesis that recombinant proteins may provide a useful adjunct to current diagnostic testing for Lyme borreliosis.

Original languageEnglish (US)
Pages (from-to)1348-1355
Number of pages8
JournalClinical and Diagnostic Laboratory Immunology
Volume9
Issue number6
DOIs
StatePublished - Nov 2002
Externally publishedYes

Fingerprint

Immunosorbents
Lyme Disease
Humoral Immunity
Recombinant Proteins
Immunoblotting
Primates
Assays
Enzyme-Linked Immunosorbent Assay
Antibody Formation
Immunoglobulin M
Antibodies
Immunoglobulin G
Enzymes
Proteins
Spirochaetales
Borrelia burgdorferi
Densitometry
Infection
Macaca mulatta
Dexamethasone

ASJC Scopus subject areas

  • Microbiology (medical)
  • Immunology and Allergy
  • Clinical Biochemistry
  • Immunology

Cite this

@article{74e214e2253448df845ceddb0127e282,
title = "Humoral immune response associated with Lyme borreliosis in nonhuman primates: Analysis by immunoblotting and enzyme-linked immunosorbent assay with sonicates or recombinant proteins",
abstract = "The immune response to Borrelia burgdorferi, the causative agent of Lyme disease, is complex. We studied the immunoglobulin M (IgM) and IgG antibody response to N40Br, a sensu stricto strain, in the rhesus macaque-(nonhuman primate [NHP]) model of infection to identify the spirochetal protein targets of specific antibody. Antigens used in enzyme-linked immunosorbent assays were whole-cell sonicates of the spirochete and recombinant proteins of B. burgdorferi. Immunoblotting with a commercially available strip and subsequent quantitative densitometry of the bands were also used. Sera from four different groups of NHPs were used: immunocompetent, transiently immunosuppressed, extended immunosuppressed, and uninfected. In immunocompetent and transiently immunosuppressed NHPs, there was a strong IgM and IgG response. Major proteins for the early IgM response were P39 and P41 and recombinant BmpA and OspC. Major proteins for the later IgG response were P39, P41, P18, P60, P66, and recombinant BmpA and DbpA. There was no significant response in the NHPs to recombinant OspA or to Arp, a 37-kDa protein that elicits an antibody response during infection in mice. Most antibody responses, except for that to DbpA, were markedly diminished by prolonged dexamethasone treatment. This study supports the hypothesis that recombinant proteins may provide a useful adjunct to current diagnostic testing for Lyme borreliosis.",
author = "Pachner, {A. R.} and D. Dail and L. Li and L. Gurey and S. Feng and Emir Hodzic and Barthold, {Stephen W}",
year = "2002",
month = "11",
doi = "10.1128/CDLI.9.6.1348-1355.2002",
language = "English (US)",
volume = "9",
pages = "1348--1355",
journal = "Clinical and Vaccine Immunology",
issn = "1556-6811",
publisher = "American Society for Microbiology",
number = "6",

}

TY - JOUR

T1 - Humoral immune response associated with Lyme borreliosis in nonhuman primates

T2 - Analysis by immunoblotting and enzyme-linked immunosorbent assay with sonicates or recombinant proteins

AU - Pachner, A. R.

AU - Dail, D.

AU - Li, L.

AU - Gurey, L.

AU - Feng, S.

AU - Hodzic, Emir

AU - Barthold, Stephen W

PY - 2002/11

Y1 - 2002/11

N2 - The immune response to Borrelia burgdorferi, the causative agent of Lyme disease, is complex. We studied the immunoglobulin M (IgM) and IgG antibody response to N40Br, a sensu stricto strain, in the rhesus macaque-(nonhuman primate [NHP]) model of infection to identify the spirochetal protein targets of specific antibody. Antigens used in enzyme-linked immunosorbent assays were whole-cell sonicates of the spirochete and recombinant proteins of B. burgdorferi. Immunoblotting with a commercially available strip and subsequent quantitative densitometry of the bands were also used. Sera from four different groups of NHPs were used: immunocompetent, transiently immunosuppressed, extended immunosuppressed, and uninfected. In immunocompetent and transiently immunosuppressed NHPs, there was a strong IgM and IgG response. Major proteins for the early IgM response were P39 and P41 and recombinant BmpA and OspC. Major proteins for the later IgG response were P39, P41, P18, P60, P66, and recombinant BmpA and DbpA. There was no significant response in the NHPs to recombinant OspA or to Arp, a 37-kDa protein that elicits an antibody response during infection in mice. Most antibody responses, except for that to DbpA, were markedly diminished by prolonged dexamethasone treatment. This study supports the hypothesis that recombinant proteins may provide a useful adjunct to current diagnostic testing for Lyme borreliosis.

AB - The immune response to Borrelia burgdorferi, the causative agent of Lyme disease, is complex. We studied the immunoglobulin M (IgM) and IgG antibody response to N40Br, a sensu stricto strain, in the rhesus macaque-(nonhuman primate [NHP]) model of infection to identify the spirochetal protein targets of specific antibody. Antigens used in enzyme-linked immunosorbent assays were whole-cell sonicates of the spirochete and recombinant proteins of B. burgdorferi. Immunoblotting with a commercially available strip and subsequent quantitative densitometry of the bands were also used. Sera from four different groups of NHPs were used: immunocompetent, transiently immunosuppressed, extended immunosuppressed, and uninfected. In immunocompetent and transiently immunosuppressed NHPs, there was a strong IgM and IgG response. Major proteins for the early IgM response were P39 and P41 and recombinant BmpA and OspC. Major proteins for the later IgG response were P39, P41, P18, P60, P66, and recombinant BmpA and DbpA. There was no significant response in the NHPs to recombinant OspA or to Arp, a 37-kDa protein that elicits an antibody response during infection in mice. Most antibody responses, except for that to DbpA, were markedly diminished by prolonged dexamethasone treatment. This study supports the hypothesis that recombinant proteins may provide a useful adjunct to current diagnostic testing for Lyme borreliosis.

UR - http://www.scopus.com/inward/record.url?scp=0036842659&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0036842659&partnerID=8YFLogxK

U2 - 10.1128/CDLI.9.6.1348-1355.2002

DO - 10.1128/CDLI.9.6.1348-1355.2002

M3 - Article

C2 - 12414773

AN - SCOPUS:0036842659

VL - 9

SP - 1348

EP - 1355

JO - Clinical and Vaccine Immunology

JF - Clinical and Vaccine Immunology

SN - 1556-6811

IS - 6

ER -