Humic acid inhibits HBV-induced autophagosome formation and induces apoptosis in HBV-transfected Hep G2 cells

Kishor Pant, Ajay K. Yadav, Parul Gupta, Abhishek Singh Rathore, Baibaswata Nayak, Senthil K. Venugopal

Research output: Contribution to journalArticle

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Abstract

Hepatitis B Virus (HBV) utilizes several mechanisms to survive in the host cells and one of the main pathways being autophagosome formation. Humic acid (HA), one of the major components of Mineral pitch, is an Ayurvedic medicinal food, commonly used by the people of the Himalayan regions of Nepal and India for various body ailments. We hypothesized that HA could induce cell death and inhibit HBV-induced autophagy in hepatic cells. Incubation of Hep G2.2.1.5 cells (HepG2 cells stably expressing HBV) with HA (100 μM) inhibited both cell proliferation and autophagosome formation significantly, while apoptosis induction was enhanced. Western blot results showed that HA incubation resulted in decreased levels of beclin-1, SIRT-1 and c-myc, while caspase-3 and β-catenin expression were up-regulated. Western blot results showed that HA significantly inhibited the expression of HBx (3-fold with 50 μM and 5-fold with 100 μM) compared to control cells. When HA was incubated with HBx-transfected Hep G2 cells, HBx-induced autophagosome formation and beclin-1 levels were decreased. These data showed that HA induced apoptosis and inhibited HBV-induced autophagosome formation and proliferation in hepatoma cells.

Original languageEnglish (US)
Article number34496
JournalScientific Reports
Volume6
DOIs
StatePublished - Oct 6 2016
Externally publishedYes

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Humic Substances
Hep G2 Cells
Hepatitis B virus
Apoptosis
Western Blotting
Catenins
Nepal
Autophagy
Autophagosomes
Caspase 3
Minerals
India
Hepatocytes
Hepatocellular Carcinoma
Cell Death
Cell Proliferation
Food

ASJC Scopus subject areas

  • General

Cite this

Humic acid inhibits HBV-induced autophagosome formation and induces apoptosis in HBV-transfected Hep G2 cells. / Pant, Kishor; Yadav, Ajay K.; Gupta, Parul; Rathore, Abhishek Singh; Nayak, Baibaswata; Venugopal, Senthil K.

In: Scientific Reports, Vol. 6, 34496, 06.10.2016.

Research output: Contribution to journalArticle

Pant, Kishor ; Yadav, Ajay K. ; Gupta, Parul ; Rathore, Abhishek Singh ; Nayak, Baibaswata ; Venugopal, Senthil K. / Humic acid inhibits HBV-induced autophagosome formation and induces apoptosis in HBV-transfected Hep G2 cells. In: Scientific Reports. 2016 ; Vol. 6.
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