Human T-lymphotropic virus type 1 mitochondrion-localizing protein p13 II sensitizes Jurkat T cells to Ras-mediated apoptosis

Hajime Hiraragi, Bindhu Michael, Amrithraj Nair, Micol Silic-Benussi, Vincenzo Ciminale, Michael Dale Lairmore

Research output: Contribution to journalArticle

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Abstract

Human T-lymphotropic virus type 1 (HTLV-1) is the etiological agent of adult T-cell leukemia. In addition to typical retroviral structural and enzymatic gene products, HTLV-1 encodes unique regulatory and accessory proteins, including a singly spliced pX open reading frame II (ORF II) product, p13II. We have demonstrated that proviral clones of HTLV-1 which are mutated in pX ORF II fail to obtain typical proviral loads and antibody responses in a rabbit animal model. p13II localizes to mitochondria and reduces cell growth and tumorigenicity in mice, but its function in human lymphocytes remains undetermined. For this study, we analyzed the functional properties of Jurkat T cells expressing p13II, using both transient and stable expression vectors. Our data indicate that p13II- expressing Jurkat T cells are sensitive to caspase-dependent, ceramide- and FasL-induced apoptosis. p13II-expressing Jurkat T cells also exhibited reduced proliferation when cultured at a high density. Furthermore, preincubation of the p13II-expressing cells with a farnesyl transferase inhibitor, which blocks the posttranslational modification of Ras, markedly reduced FasL-induced apoptosis, indicating the participation of the Ras pathway in p13II's influence on lymphocyte survival. Our data are the first to demonstrate that p13II alters Ras-mediated apoptosis in T lymphocytes, and they reveal a potential mechanism by which HTLV-1 alters lymphocyte proliferation.

Original languageEnglish (US)
Pages (from-to)9449-9457
Number of pages9
JournalJournal of Virology
Volume79
Issue number15
DOIs
StatePublished - Aug 2005
Externally publishedYes

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Human T-lymphotropic virus 1
Jurkat Cells
Mitochondria
mitochondria
apoptosis
T-lymphocytes
Apoptosis
T-Lymphocytes
viruses
Lymphocytes
Open Reading Frames
Proteins
proteins
open reading frames
Adult T Cell Leukemia Lymphoma
lymphocytes
Ceramides
Post Translational Protein Processing
Caspases
Transferases

ASJC Scopus subject areas

  • Immunology

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Human T-lymphotropic virus type 1 mitochondrion-localizing protein p13 II sensitizes Jurkat T cells to Ras-mediated apoptosis. / Hiraragi, Hajime; Michael, Bindhu; Nair, Amrithraj; Silic-Benussi, Micol; Ciminale, Vincenzo; Lairmore, Michael Dale.

In: Journal of Virology, Vol. 79, No. 15, 08.2005, p. 9449-9457.

Research output: Contribution to journalArticle

Hiraragi, Hajime ; Michael, Bindhu ; Nair, Amrithraj ; Silic-Benussi, Micol ; Ciminale, Vincenzo ; Lairmore, Michael Dale. / Human T-lymphotropic virus type 1 mitochondrion-localizing protein p13 II sensitizes Jurkat T cells to Ras-mediated apoptosis. In: Journal of Virology. 2005 ; Vol. 79, No. 15. pp. 9449-9457.
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