Human serum processing and analysis methods for rapid and reproducible N-glycan mass profiling

Scott R. Kronewitter, Maria Lorna A De Leoz, Kyle S. Peacock, Kelly R. McBride, Hyun Joo An, Suzanne Miyamoto, Gary S Leiserowitz, Carlito B Lebrilla

Research output: Contribution to journalArticle

59 Citations (Scopus)

Abstract

Glycans constitute a new class of compounds for biomarker discovery. Glycosylation is a common post-translational modification and is often associated with transformation to malignancy. To analyze glycans, they are released from proteins, enriched, and measured with mass spectrometry. For biomarker discovery, repeatability at every step of the process is important. Locating and minimizing the process variability is key to establishing a robust platform stable enough for biomarker discovery. Understanding the variability of the measurement devices helps understand the variability associated with the chemical processing. This report explores the potential use of methods expediting the enzymatic release of glycans such as a microwave reactor and automation of the solid-phase extraction with a robotic liquid handler. The study employs matrix-assisted laser desorption/ionization-Fourier transform ion cyclotron resonance mass spectrometry but would be suitable with any mass spectrometry method. Methods for system-wide data analysis are examined because proper metrics for evaluating the performance of glycan sample preparation procedures are not well established.

Original languageEnglish (US)
Pages (from-to)4952-4959
Number of pages8
JournalJournal of Proteome Research
Volume9
Issue number10
DOIs
StatePublished - Oct 1 2010

Fingerprint

Polysaccharides
Biomarkers
Mass spectrometry
Mass Spectrometry
Processing
Serum
Glycosylation
Cyclotrons
Cyclotron resonance
Automation
Solid Phase Extraction
Robotics
Fourier Analysis
Post Translational Protein Processing
Microwaves
Ionization
Desorption
Fourier transforms
Lasers
Ions

Keywords

  • Fourier transform ion cyclotron resonance mass spectrometry
  • glycomics
  • human serum
  • interpretation of mass spectra

ASJC Scopus subject areas

  • Biochemistry
  • Chemistry(all)

Cite this

Kronewitter, S. R., De Leoz, M. L. A., Peacock, K. S., McBride, K. R., An, H. J., Miyamoto, S., ... Lebrilla, C. B. (2010). Human serum processing and analysis methods for rapid and reproducible N-glycan mass profiling. Journal of Proteome Research, 9(10), 4952-4959. https://doi.org/10.1021/pr100202a

Human serum processing and analysis methods for rapid and reproducible N-glycan mass profiling. / Kronewitter, Scott R.; De Leoz, Maria Lorna A; Peacock, Kyle S.; McBride, Kelly R.; An, Hyun Joo; Miyamoto, Suzanne; Leiserowitz, Gary S; Lebrilla, Carlito B.

In: Journal of Proteome Research, Vol. 9, No. 10, 01.10.2010, p. 4952-4959.

Research output: Contribution to journalArticle

Kronewitter, SR, De Leoz, MLA, Peacock, KS, McBride, KR, An, HJ, Miyamoto, S, Leiserowitz, GS & Lebrilla, CB 2010, 'Human serum processing and analysis methods for rapid and reproducible N-glycan mass profiling', Journal of Proteome Research, vol. 9, no. 10, pp. 4952-4959. https://doi.org/10.1021/pr100202a
Kronewitter SR, De Leoz MLA, Peacock KS, McBride KR, An HJ, Miyamoto S et al. Human serum processing and analysis methods for rapid and reproducible N-glycan mass profiling. Journal of Proteome Research. 2010 Oct 1;9(10):4952-4959. https://doi.org/10.1021/pr100202a
Kronewitter, Scott R. ; De Leoz, Maria Lorna A ; Peacock, Kyle S. ; McBride, Kelly R. ; An, Hyun Joo ; Miyamoto, Suzanne ; Leiserowitz, Gary S ; Lebrilla, Carlito B. / Human serum processing and analysis methods for rapid and reproducible N-glycan mass profiling. In: Journal of Proteome Research. 2010 ; Vol. 9, No. 10. pp. 4952-4959.
@article{227f64935e3347748a0b47e9c30cb6fa,
title = "Human serum processing and analysis methods for rapid and reproducible N-glycan mass profiling",
abstract = "Glycans constitute a new class of compounds for biomarker discovery. Glycosylation is a common post-translational modification and is often associated with transformation to malignancy. To analyze glycans, they are released from proteins, enriched, and measured with mass spectrometry. For biomarker discovery, repeatability at every step of the process is important. Locating and minimizing the process variability is key to establishing a robust platform stable enough for biomarker discovery. Understanding the variability of the measurement devices helps understand the variability associated with the chemical processing. This report explores the potential use of methods expediting the enzymatic release of glycans such as a microwave reactor and automation of the solid-phase extraction with a robotic liquid handler. The study employs matrix-assisted laser desorption/ionization-Fourier transform ion cyclotron resonance mass spectrometry but would be suitable with any mass spectrometry method. Methods for system-wide data analysis are examined because proper metrics for evaluating the performance of glycan sample preparation procedures are not well established.",
keywords = "Fourier transform ion cyclotron resonance mass spectrometry, glycomics, human serum, interpretation of mass spectra",
author = "Kronewitter, {Scott R.} and {De Leoz}, {Maria Lorna A} and Peacock, {Kyle S.} and McBride, {Kelly R.} and An, {Hyun Joo} and Suzanne Miyamoto and Leiserowitz, {Gary S} and Lebrilla, {Carlito B}",
year = "2010",
month = "10",
day = "1",
doi = "10.1021/pr100202a",
language = "English (US)",
volume = "9",
pages = "4952--4959",
journal = "Journal of Proteome Research",
issn = "1535-3893",
publisher = "American Chemical Society",
number = "10",

}

TY - JOUR

T1 - Human serum processing and analysis methods for rapid and reproducible N-glycan mass profiling

AU - Kronewitter, Scott R.

AU - De Leoz, Maria Lorna A

AU - Peacock, Kyle S.

AU - McBride, Kelly R.

AU - An, Hyun Joo

AU - Miyamoto, Suzanne

AU - Leiserowitz, Gary S

AU - Lebrilla, Carlito B

PY - 2010/10/1

Y1 - 2010/10/1

N2 - Glycans constitute a new class of compounds for biomarker discovery. Glycosylation is a common post-translational modification and is often associated with transformation to malignancy. To analyze glycans, they are released from proteins, enriched, and measured with mass spectrometry. For biomarker discovery, repeatability at every step of the process is important. Locating and minimizing the process variability is key to establishing a robust platform stable enough for biomarker discovery. Understanding the variability of the measurement devices helps understand the variability associated with the chemical processing. This report explores the potential use of methods expediting the enzymatic release of glycans such as a microwave reactor and automation of the solid-phase extraction with a robotic liquid handler. The study employs matrix-assisted laser desorption/ionization-Fourier transform ion cyclotron resonance mass spectrometry but would be suitable with any mass spectrometry method. Methods for system-wide data analysis are examined because proper metrics for evaluating the performance of glycan sample preparation procedures are not well established.

AB - Glycans constitute a new class of compounds for biomarker discovery. Glycosylation is a common post-translational modification and is often associated with transformation to malignancy. To analyze glycans, they are released from proteins, enriched, and measured with mass spectrometry. For biomarker discovery, repeatability at every step of the process is important. Locating and minimizing the process variability is key to establishing a robust platform stable enough for biomarker discovery. Understanding the variability of the measurement devices helps understand the variability associated with the chemical processing. This report explores the potential use of methods expediting the enzymatic release of glycans such as a microwave reactor and automation of the solid-phase extraction with a robotic liquid handler. The study employs matrix-assisted laser desorption/ionization-Fourier transform ion cyclotron resonance mass spectrometry but would be suitable with any mass spectrometry method. Methods for system-wide data analysis are examined because proper metrics for evaluating the performance of glycan sample preparation procedures are not well established.

KW - Fourier transform ion cyclotron resonance mass spectrometry

KW - glycomics

KW - human serum

KW - interpretation of mass spectra

UR - http://www.scopus.com/inward/record.url?scp=77957369944&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=77957369944&partnerID=8YFLogxK

U2 - 10.1021/pr100202a

DO - 10.1021/pr100202a

M3 - Article

C2 - 20698584

AN - SCOPUS:77957369944

VL - 9

SP - 4952

EP - 4959

JO - Journal of Proteome Research

JF - Journal of Proteome Research

SN - 1535-3893

IS - 10

ER -