Human recombinant interleukin-2125 induced in vitro proliferation of equine, caprine, ovine, canine and feline peripheral blood lymphocytes

Brad W. Fenwick, Caroline E. Schore, Bennie Osburn

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Equine, caprine, ovine, canine and feline peripheral blood lymphocytes were evaluated in a short term dose-response study for their in vitro blastogenic responsiveness to human recombinant interleukin-2125 (HrIL-2125) alone or in combination with phytohemagglutinin-P, concanavalin-A, and pokeweed mitogen. HrIL-2125 induced lymphocyte proliferation in all of the animals tested. The magnitude of the proliferative response varied among the species of animal tested. In all cases the proliferative response was dependent on the concentration of HrIL-2125. HrIL-2125 at a minimum concentration of 102 Cetus Units (CU)/ml produced a significant proliferative response in isolated horse, goat and sheep lymphocytes. In cat and dog lymphocytes, a concentration of 103 CU/ml was necessary to induce a significant proliferative response. Maximal lymphocyte proliferation was reached in horses and sheep at a concentration of 104 CU/ml of HrIL-2125. In goats, cats, and dogs a maximum proliferative response was found to be at a concentration equal to or greater than 104 CU/ml of HrIL-2. Co-stimulation of lymphocytes with mitogens and submaximal concentrations of HrIL-2125 (10 CU/ml) induced a synergistic proliferative response which in nearly all cases was significantly greater (P < 0.05) than the arithmetic sum of the responses induced by the same concentration of the mitogens and HrIL-2125 alone. The two exceptions were co-stimulation of feline lymphocytes with concanavalin-A and co-stimulation of canine lymphocytes with pokeweed mitogen.

Original languageEnglish (US)
Pages (from-to)51-60
Number of pages10
JournalComparative Immunology, Microbiology and Infectious Diseases
Volume11
Issue number1
DOIs
StatePublished - Jan 1 1988

Fingerprint

Interleukins
Felidae
interleukins
Horses
Canidae
Sheep
lymphocytes
goats
Lymphocytes
cats
horses
sheep
dogs
blood
lymphocyte proliferation
Lymphocyte Activation
Pokeweed Mitogens
Phytolacca americana
concanavalin A
Concanavalin A

Keywords

  • cat
  • dog
  • goat
  • horse
  • immunology
  • Interleukin-2
  • lymphocyte
  • lymphokine
  • sheep

ASJC Scopus subject areas

  • Immunology and Allergy
  • Microbiology
  • Immunology
  • veterinary(all)
  • Infectious Diseases

Cite this

@article{580014bf9f144188b3410972eb687e6a,
title = "Human recombinant interleukin-2125 induced in vitro proliferation of equine, caprine, ovine, canine and feline peripheral blood lymphocytes",
abstract = "Equine, caprine, ovine, canine and feline peripheral blood lymphocytes were evaluated in a short term dose-response study for their in vitro blastogenic responsiveness to human recombinant interleukin-2125 (HrIL-2125) alone or in combination with phytohemagglutinin-P, concanavalin-A, and pokeweed mitogen. HrIL-2125 induced lymphocyte proliferation in all of the animals tested. The magnitude of the proliferative response varied among the species of animal tested. In all cases the proliferative response was dependent on the concentration of HrIL-2125. HrIL-2125 at a minimum concentration of 102 Cetus Units (CU)/ml produced a significant proliferative response in isolated horse, goat and sheep lymphocytes. In cat and dog lymphocytes, a concentration of 103 CU/ml was necessary to induce a significant proliferative response. Maximal lymphocyte proliferation was reached in horses and sheep at a concentration of 104 CU/ml of HrIL-2125. In goats, cats, and dogs a maximum proliferative response was found to be at a concentration equal to or greater than 104 CU/ml of HrIL-2. Co-stimulation of lymphocytes with mitogens and submaximal concentrations of HrIL-2125 (10 CU/ml) induced a synergistic proliferative response which in nearly all cases was significantly greater (P < 0.05) than the arithmetic sum of the responses induced by the same concentration of the mitogens and HrIL-2125 alone. The two exceptions were co-stimulation of feline lymphocytes with concanavalin-A and co-stimulation of canine lymphocytes with pokeweed mitogen.",
keywords = "cat, dog, goat, horse, immunology, Interleukin-2, lymphocyte, lymphokine, sheep",
author = "Fenwick, {Brad W.} and Schore, {Caroline E.} and Bennie Osburn",
year = "1988",
month = "1",
day = "1",
doi = "10.1016/0147-9571(88)90008-2",
language = "English (US)",
volume = "11",
pages = "51--60",
journal = "Comparative Immunology, Microbiology and Infectious Diseases",
issn = "0147-9571",
publisher = "Elsevier Limited",
number = "1",

}

TY - JOUR

T1 - Human recombinant interleukin-2125 induced in vitro proliferation of equine, caprine, ovine, canine and feline peripheral blood lymphocytes

AU - Fenwick, Brad W.

AU - Schore, Caroline E.

AU - Osburn, Bennie

PY - 1988/1/1

Y1 - 1988/1/1

N2 - Equine, caprine, ovine, canine and feline peripheral blood lymphocytes were evaluated in a short term dose-response study for their in vitro blastogenic responsiveness to human recombinant interleukin-2125 (HrIL-2125) alone or in combination with phytohemagglutinin-P, concanavalin-A, and pokeweed mitogen. HrIL-2125 induced lymphocyte proliferation in all of the animals tested. The magnitude of the proliferative response varied among the species of animal tested. In all cases the proliferative response was dependent on the concentration of HrIL-2125. HrIL-2125 at a minimum concentration of 102 Cetus Units (CU)/ml produced a significant proliferative response in isolated horse, goat and sheep lymphocytes. In cat and dog lymphocytes, a concentration of 103 CU/ml was necessary to induce a significant proliferative response. Maximal lymphocyte proliferation was reached in horses and sheep at a concentration of 104 CU/ml of HrIL-2125. In goats, cats, and dogs a maximum proliferative response was found to be at a concentration equal to or greater than 104 CU/ml of HrIL-2. Co-stimulation of lymphocytes with mitogens and submaximal concentrations of HrIL-2125 (10 CU/ml) induced a synergistic proliferative response which in nearly all cases was significantly greater (P < 0.05) than the arithmetic sum of the responses induced by the same concentration of the mitogens and HrIL-2125 alone. The two exceptions were co-stimulation of feline lymphocytes with concanavalin-A and co-stimulation of canine lymphocytes with pokeweed mitogen.

AB - Equine, caprine, ovine, canine and feline peripheral blood lymphocytes were evaluated in a short term dose-response study for their in vitro blastogenic responsiveness to human recombinant interleukin-2125 (HrIL-2125) alone or in combination with phytohemagglutinin-P, concanavalin-A, and pokeweed mitogen. HrIL-2125 induced lymphocyte proliferation in all of the animals tested. The magnitude of the proliferative response varied among the species of animal tested. In all cases the proliferative response was dependent on the concentration of HrIL-2125. HrIL-2125 at a minimum concentration of 102 Cetus Units (CU)/ml produced a significant proliferative response in isolated horse, goat and sheep lymphocytes. In cat and dog lymphocytes, a concentration of 103 CU/ml was necessary to induce a significant proliferative response. Maximal lymphocyte proliferation was reached in horses and sheep at a concentration of 104 CU/ml of HrIL-2125. In goats, cats, and dogs a maximum proliferative response was found to be at a concentration equal to or greater than 104 CU/ml of HrIL-2. Co-stimulation of lymphocytes with mitogens and submaximal concentrations of HrIL-2125 (10 CU/ml) induced a synergistic proliferative response which in nearly all cases was significantly greater (P < 0.05) than the arithmetic sum of the responses induced by the same concentration of the mitogens and HrIL-2125 alone. The two exceptions were co-stimulation of feline lymphocytes with concanavalin-A and co-stimulation of canine lymphocytes with pokeweed mitogen.

KW - cat

KW - dog

KW - goat

KW - horse

KW - immunology

KW - Interleukin-2

KW - lymphocyte

KW - lymphokine

KW - sheep

UR - http://www.scopus.com/inward/record.url?scp=0023912062&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0023912062&partnerID=8YFLogxK

U2 - 10.1016/0147-9571(88)90008-2

DO - 10.1016/0147-9571(88)90008-2

M3 - Article

C2 - 3260166

AN - SCOPUS:0023912062

VL - 11

SP - 51

EP - 60

JO - Comparative Immunology, Microbiology and Infectious Diseases

JF - Comparative Immunology, Microbiology and Infectious Diseases

SN - 0147-9571

IS - 1

ER -