Human Rad51 protein displays enhanced homologous pairing of DNA sequences resembling those at genetically unstable loci

Erica M. Seitz, Stephen C. Kowalczykowski

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

DNA strand exchange, the central step of homologous recombination, is considered to occur approximately independently of DNA sequence content. However, certain prokaryotic and eukaryotic genomic loci display either an enhanced or reduced frequency of genetic exchange. Here we show that the Homo sapiens DNA strand exchange protein, HsRad51, shows a preference for binding to single-stranded DNA sequences primarily rich in G-residues and poor in A- and C-residues, and that these DNA sequences manifest enhanced HsRad51 protein-dependent homologous pairing. Both of these properties are common to all DNA strand exchange proteins examined thus far. These preferred DNA pairing sequences resemble those found at genetic loci in human cells that cause genomic instability and lead to genetic diseases.

Original languageEnglish (US)
Pages (from-to)2847-2852
Number of pages6
JournalNucleic Acids Research
Volume34
Issue number10
DOIs
StatePublished - 2006

Fingerprint

DNA
Proteins
Inborn Genetic Diseases
Genetic Loci
Genomic Instability
Single-Stranded DNA
Homologous Recombination

ASJC Scopus subject areas

  • Genetics

Cite this

Human Rad51 protein displays enhanced homologous pairing of DNA sequences resembling those at genetically unstable loci. / Seitz, Erica M.; Kowalczykowski, Stephen C.

In: Nucleic Acids Research, Vol. 34, No. 10, 2006, p. 2847-2852.

Research output: Contribution to journalArticle

@article{de1a5d1bc2f04a4f8a98a21c2ef971bd,
title = "Human Rad51 protein displays enhanced homologous pairing of DNA sequences resembling those at genetically unstable loci",
abstract = "DNA strand exchange, the central step of homologous recombination, is considered to occur approximately independently of DNA sequence content. However, certain prokaryotic and eukaryotic genomic loci display either an enhanced or reduced frequency of genetic exchange. Here we show that the Homo sapiens DNA strand exchange protein, HsRad51, shows a preference for binding to single-stranded DNA sequences primarily rich in G-residues and poor in A- and C-residues, and that these DNA sequences manifest enhanced HsRad51 protein-dependent homologous pairing. Both of these properties are common to all DNA strand exchange proteins examined thus far. These preferred DNA pairing sequences resemble those found at genetic loci in human cells that cause genomic instability and lead to genetic diseases.",
author = "Seitz, {Erica M.} and Kowalczykowski, {Stephen C.}",
year = "2006",
doi = "10.1093/nar/gkl355",
language = "English (US)",
volume = "34",
pages = "2847--2852",
journal = "Nucleic Acids Research",
issn = "0305-1048",
publisher = "Oxford University Press",
number = "10",

}

TY - JOUR

T1 - Human Rad51 protein displays enhanced homologous pairing of DNA sequences resembling those at genetically unstable loci

AU - Seitz, Erica M.

AU - Kowalczykowski, Stephen C.

PY - 2006

Y1 - 2006

N2 - DNA strand exchange, the central step of homologous recombination, is considered to occur approximately independently of DNA sequence content. However, certain prokaryotic and eukaryotic genomic loci display either an enhanced or reduced frequency of genetic exchange. Here we show that the Homo sapiens DNA strand exchange protein, HsRad51, shows a preference for binding to single-stranded DNA sequences primarily rich in G-residues and poor in A- and C-residues, and that these DNA sequences manifest enhanced HsRad51 protein-dependent homologous pairing. Both of these properties are common to all DNA strand exchange proteins examined thus far. These preferred DNA pairing sequences resemble those found at genetic loci in human cells that cause genomic instability and lead to genetic diseases.

AB - DNA strand exchange, the central step of homologous recombination, is considered to occur approximately independently of DNA sequence content. However, certain prokaryotic and eukaryotic genomic loci display either an enhanced or reduced frequency of genetic exchange. Here we show that the Homo sapiens DNA strand exchange protein, HsRad51, shows a preference for binding to single-stranded DNA sequences primarily rich in G-residues and poor in A- and C-residues, and that these DNA sequences manifest enhanced HsRad51 protein-dependent homologous pairing. Both of these properties are common to all DNA strand exchange proteins examined thus far. These preferred DNA pairing sequences resemble those found at genetic loci in human cells that cause genomic instability and lead to genetic diseases.

UR - http://www.scopus.com/inward/record.url?scp=33744542377&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33744542377&partnerID=8YFLogxK

U2 - 10.1093/nar/gkl355

DO - 10.1093/nar/gkl355

M3 - Article

C2 - 16723430

AN - SCOPUS:33744542377

VL - 34

SP - 2847

EP - 2852

JO - Nucleic Acids Research

JF - Nucleic Acids Research

SN - 0305-1048

IS - 10

ER -