Human Myoblast and Mesenchymal Stem Cell Interactions Visualized by Videomicroscopy

Amanda B. Black, Heather Dahlenburg, Karen Pepper, Catherine Nacey, Suzanne Pontow, Maggie Kuhn, Peter C Belafsky, Jan Nolta

Research output: Contribution to journalArticlepeer-review

2 Scopus citations

Abstract

Muscle-derived progenitor cell (myoblast) therapy has promise for the treatment of denervated, weakened, and fibrotic muscle. The best methods for injecting myoblasts to promote fusion and retention have yet to be determined, however. Mesenchymal stem/stromal cells have also been reported to have beneficial effects in restoring damaged tissue, through increasing vascularization and reducing inflammation. The interactions between human primary skeletal myoblasts and bone marrow-derived mesenchymal stem/stromal cells were examined using time-lapse images put into video format. Of interest, there is a high degree of cell-to-cell interaction with microparticles transferring between both cell types, and formation of nanotubules to bridge cytoplasmic contents between the two types of cell. This model provides an in vitro platform for examining mechanisms for cell-to-cell interaction preceding myoblast fusion.

Original languageEnglish (US)
Pages (from-to)193-196
Number of pages4
JournalHuman gene therapy methods
Volume26
Issue number6
DOIs
StatePublished - Dec 1 2015

ASJC Scopus subject areas

  • Molecular Medicine
  • Applied Microbiology and Biotechnology
  • Genetics(clinical)
  • Genetics
  • Pharmacology

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