Abstract
Background: Chikungunya (CHIK) virus is a mosquito-transmitted alphavirus that causes in humans an acute infection characterised by fever, polyarthralgia, head-ache, and myalgia. Since 2005, the emergence of CHIK virus was associated with an unprecedented magnitude outbreak of CHIK disease in the Indian Ocean. Clinically, this outbreak was characterized by invalidating poly-arthralgia, with myalgia being reported in 97.7% of cases. Since the cellular targets of CHIK virus in humans are unknown, we studied the pathogenic events and targets of CHIK infection in skeletal muscle. Methodology/Principal Findings: Immunohistology on muscle biopsies from two CHIK virus-infected patients with myositic syndrome showed that viral antigens were found exclusively inside skeletal muscle progenitor cells (designed as satelllite cells), and not in muscle fibers. To evaluate the ability of CHIK virus to replicate in human satellite cells, we assessed virus infection on primary human muscle cells; viral growth was observed in CHIK virus-infected satellite cells with a cytopathic effect, whereas myotubes were essentially refractory to infection. Conclusions/Significance: This report provides new insights into CHIK virus pathogenesis, since it is the first to identify a cellular target of CHIK virus in humans and to report a selective infection of muscle satellite cells by a viral agent in humans.
| Original language | English (US) |
|---|---|
| Article number | e527 |
| Journal | PLoS One |
| Volume | 2 |
| Issue number | 6 |
| DOIs | |
| State | Published - 2007 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Agricultural and Biological Sciences(all)
- Biochemistry, Genetics and Molecular Biology(all)
- Medicine(all)
Cite this
Human Muscle Satellite Cells as Targets of Chikungunya Virus Infection. / Ozden, Simona; Huerre, Michel; Riviere, Jean Pierre; Schneider, Lark L; Afonso, Philippe V.; Mouly, Vincent; de Monredon, Jean; Roger, Jean Christophe; El Amrani, Mohamed; Yvin, Jean Luc; Jaffar, Marie Christine; Frenkiel, Marie Pascale; Sourisseau, Marion; Schwartz, Olivier; Butler-Browne, Gillian; Desprès, Philippe; Gessain, Antoine; Ceccaldi, Pierre Emmanuel.
In: PLoS One, Vol. 2, No. 6, e527, 2007.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Human Muscle Satellite Cells as Targets of Chikungunya Virus Infection
AU - Ozden, Simona
AU - Huerre, Michel
AU - Riviere, Jean Pierre
AU - Schneider, Lark L
AU - Afonso, Philippe V.
AU - Mouly, Vincent
AU - de Monredon, Jean
AU - Roger, Jean Christophe
AU - El Amrani, Mohamed
AU - Yvin, Jean Luc
AU - Jaffar, Marie Christine
AU - Frenkiel, Marie Pascale
AU - Sourisseau, Marion
AU - Schwartz, Olivier
AU - Butler-Browne, Gillian
AU - Desprès, Philippe
AU - Gessain, Antoine
AU - Ceccaldi, Pierre Emmanuel
PY - 2007
Y1 - 2007
N2 - Background: Chikungunya (CHIK) virus is a mosquito-transmitted alphavirus that causes in humans an acute infection characterised by fever, polyarthralgia, head-ache, and myalgia. Since 2005, the emergence of CHIK virus was associated with an unprecedented magnitude outbreak of CHIK disease in the Indian Ocean. Clinically, this outbreak was characterized by invalidating poly-arthralgia, with myalgia being reported in 97.7% of cases. Since the cellular targets of CHIK virus in humans are unknown, we studied the pathogenic events and targets of CHIK infection in skeletal muscle. Methodology/Principal Findings: Immunohistology on muscle biopsies from two CHIK virus-infected patients with myositic syndrome showed that viral antigens were found exclusively inside skeletal muscle progenitor cells (designed as satelllite cells), and not in muscle fibers. To evaluate the ability of CHIK virus to replicate in human satellite cells, we assessed virus infection on primary human muscle cells; viral growth was observed in CHIK virus-infected satellite cells with a cytopathic effect, whereas myotubes were essentially refractory to infection. Conclusions/Significance: This report provides new insights into CHIK virus pathogenesis, since it is the first to identify a cellular target of CHIK virus in humans and to report a selective infection of muscle satellite cells by a viral agent in humans.
AB - Background: Chikungunya (CHIK) virus is a mosquito-transmitted alphavirus that causes in humans an acute infection characterised by fever, polyarthralgia, head-ache, and myalgia. Since 2005, the emergence of CHIK virus was associated with an unprecedented magnitude outbreak of CHIK disease in the Indian Ocean. Clinically, this outbreak was characterized by invalidating poly-arthralgia, with myalgia being reported in 97.7% of cases. Since the cellular targets of CHIK virus in humans are unknown, we studied the pathogenic events and targets of CHIK infection in skeletal muscle. Methodology/Principal Findings: Immunohistology on muscle biopsies from two CHIK virus-infected patients with myositic syndrome showed that viral antigens were found exclusively inside skeletal muscle progenitor cells (designed as satelllite cells), and not in muscle fibers. To evaluate the ability of CHIK virus to replicate in human satellite cells, we assessed virus infection on primary human muscle cells; viral growth was observed in CHIK virus-infected satellite cells with a cytopathic effect, whereas myotubes were essentially refractory to infection. Conclusions/Significance: This report provides new insights into CHIK virus pathogenesis, since it is the first to identify a cellular target of CHIK virus in humans and to report a selective infection of muscle satellite cells by a viral agent in humans.
UR - http://www.scopus.com/inward/record.url?scp=38049021392&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=38049021392&partnerID=8YFLogxK
U2 - 10.1371/journal.pone.0000527
DO - 10.1371/journal.pone.0000527
M3 - Article
C2 - 17565380
AN - SCOPUS:38049021392
VL - 2
JO - PLoS One
JF - PLoS One
SN - 1932-6203
IS - 6
M1 - e527
ER -