Abstract
MS2B6, a human monoclonal antibody derived from a patient with advanced ovarian cancer, has been used to study the distribution and characteristics of its target antigen. The MS2B6 antigen was detected by immunoperoxidase studies in 41 of 41 epithelial ovarian cancers and in the majority of nonovarian adenocarcinomas. Among normal tissues the MS2B6 antigen was found in the adult epithelia of the fallopian tube, endometrium, endocervix, colon, bronchus, breast, sweat duct, and large renal ducts. No detectable antigen was found in peritoneal epithelia, tissue stromal cells, spleen, thymus, or blood-borne cells. Immunoblotting analysis showed that the MS2B6 epitope resides on polypeptides of 38, 44, and 60 kd. The cellular location of the MS2B6 antigen was studied with immunoperoxidase and immunofluorescent staining and immunoelectronmicroscopy of ovarian cancer ascites tumor cells. The results suggest that in ascites tumor cells the MS2B6 antigen is located in a layer of the peripheral cytoplasm beginning just below the cell membrane. MS2B6 may be useful as an imaging or therapeutic agent.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 634-645 |
| Number of pages | 12 |
| Journal | American Journal of Obstetrics and Gynecology |
| Volume | 166 |
| Issue number | 2 |
| DOIs | |
| State | Published - 1992 |
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Keywords
- Human monoclonal antibodies
- immunoperoxidase
- ovarian cancer
ASJC Scopus subject areas
- Medicine(all)
- Obstetrics and Gynecology
Cite this
Human monoclonal antibody recognizing an antigen associated with ovarian and other adenocarcinomas. / Smith, Lloyd H; Yin, Amy; Glasky, Michelle S.; Tyler, Nancy; Robles, Mariana; Foster, Chris A.; Bieber, Marcia; Teng, Nelson N H.
In: American Journal of Obstetrics and Gynecology, Vol. 166, No. 2, 1992, p. 634-645.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Human monoclonal antibody recognizing an antigen associated with ovarian and other adenocarcinomas
AU - Smith, Lloyd H
AU - Yin, Amy
AU - Glasky, Michelle S.
AU - Tyler, Nancy
AU - Robles, Mariana
AU - Foster, Chris A.
AU - Bieber, Marcia
AU - Teng, Nelson N H
PY - 1992
Y1 - 1992
N2 - MS2B6, a human monoclonal antibody derived from a patient with advanced ovarian cancer, has been used to study the distribution and characteristics of its target antigen. The MS2B6 antigen was detected by immunoperoxidase studies in 41 of 41 epithelial ovarian cancers and in the majority of nonovarian adenocarcinomas. Among normal tissues the MS2B6 antigen was found in the adult epithelia of the fallopian tube, endometrium, endocervix, colon, bronchus, breast, sweat duct, and large renal ducts. No detectable antigen was found in peritoneal epithelia, tissue stromal cells, spleen, thymus, or blood-borne cells. Immunoblotting analysis showed that the MS2B6 epitope resides on polypeptides of 38, 44, and 60 kd. The cellular location of the MS2B6 antigen was studied with immunoperoxidase and immunofluorescent staining and immunoelectronmicroscopy of ovarian cancer ascites tumor cells. The results suggest that in ascites tumor cells the MS2B6 antigen is located in a layer of the peripheral cytoplasm beginning just below the cell membrane. MS2B6 may be useful as an imaging or therapeutic agent.
AB - MS2B6, a human monoclonal antibody derived from a patient with advanced ovarian cancer, has been used to study the distribution and characteristics of its target antigen. The MS2B6 antigen was detected by immunoperoxidase studies in 41 of 41 epithelial ovarian cancers and in the majority of nonovarian adenocarcinomas. Among normal tissues the MS2B6 antigen was found in the adult epithelia of the fallopian tube, endometrium, endocervix, colon, bronchus, breast, sweat duct, and large renal ducts. No detectable antigen was found in peritoneal epithelia, tissue stromal cells, spleen, thymus, or blood-borne cells. Immunoblotting analysis showed that the MS2B6 epitope resides on polypeptides of 38, 44, and 60 kd. The cellular location of the MS2B6 antigen was studied with immunoperoxidase and immunofluorescent staining and immunoelectronmicroscopy of ovarian cancer ascites tumor cells. The results suggest that in ascites tumor cells the MS2B6 antigen is located in a layer of the peripheral cytoplasm beginning just below the cell membrane. MS2B6 may be useful as an imaging or therapeutic agent.
KW - Human monoclonal antibodies
KW - immunoperoxidase
KW - ovarian cancer
UR - http://www.scopus.com/inward/record.url?scp=0026544157&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0026544157&partnerID=8YFLogxK
U2 - 10.1016/0002-9378(92)91690-C
DO - 10.1016/0002-9378(92)91690-C
M3 - Article
C2 - 1371375
AN - SCOPUS:0026544157
VL - 166
SP - 634
EP - 645
JO - American Journal of Obstetrics and Gynecology
JF - American Journal of Obstetrics and Gynecology
SN - 0002-9378
IS - 2
ER -