Human monoclonal antibody recognizing an antigen associated with ovarian and other adenocarcinomas

Lloyd H Smith, Amy Yin, Michelle S. Glasky, Nancy Tyler, Mariana Robles, Chris A. Foster, Marcia Bieber, Nelson N H Teng

Research output: Contribution to journalArticle

6 Scopus citations

Abstract

MS2B6, a human monoclonal antibody derived from a patient with advanced ovarian cancer, has been used to study the distribution and characteristics of its target antigen. The MS2B6 antigen was detected by immunoperoxidase studies in 41 of 41 epithelial ovarian cancers and in the majority of nonovarian adenocarcinomas. Among normal tissues the MS2B6 antigen was found in the adult epithelia of the fallopian tube, endometrium, endocervix, colon, bronchus, breast, sweat duct, and large renal ducts. No detectable antigen was found in peritoneal epithelia, tissue stromal cells, spleen, thymus, or blood-borne cells. Immunoblotting analysis showed that the MS2B6 epitope resides on polypeptides of 38, 44, and 60 kd. The cellular location of the MS2B6 antigen was studied with immunoperoxidase and immunofluorescent staining and immunoelectronmicroscopy of ovarian cancer ascites tumor cells. The results suggest that in ascites tumor cells the MS2B6 antigen is located in a layer of the peripheral cytoplasm beginning just below the cell membrane. MS2B6 may be useful as an imaging or therapeutic agent.

Original languageEnglish (US)
Pages (from-to)634-645
Number of pages12
JournalAmerican Journal of Obstetrics and Gynecology
Volume166
Issue number2
DOIs
StatePublished - 1992

Keywords

  • Human monoclonal antibodies
  • immunoperoxidase
  • ovarian cancer

ASJC Scopus subject areas

  • Medicine(all)
  • Obstetrics and Gynecology

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