Abstract
Scope: Human milk exosomes provide a natural means of genetic material transfer to infants; however, the effect of gastric/pancreatic digestion milk exosomes stability and their microRNA content is largely unknown. Methods and results: We took a simulated gastric/pancreatic digestion protocol to perform in vitro digestion of milk exosomes, explore intestinal epithelial uptake, and further elucidate microRNA responses to digestion at early-, mid-, late lactation by massive parallel sequencing. Both undigested and digested exosomes enter human intestinal crypt-like cells (HIEC), with evidence of nuclear localization. We identified 288 mature microRNAs from all 24 exosome samples, and an additional 610 at low abundance. A large number of synapse development- and immune-related microRNAs were identified. hsa-miR-22-3p was the most abundant microRNA, and the top 15 microRNAs contributed ∼11% of the sequencing reads. Upon digestion, the overall microRNA abundance in human milk exosomes was stable. Conclusion: Our results for the first time reveal the survivability and complexity of human milk exosome microRNAs upon simulated gastric/pancreatic digestion, and the dynamics during lactation stages. The results suggest a previously underexplored area of infant response to genetic material in human milk exosomes.
| Original language | English (US) |
|---|---|
| Article number | 1700082 |
| Journal | Molecular Nutrition and Food Research |
| Volume | 61 |
| Issue number | 11 |
| DOIs | |
| State | Published - Nov 1 2017 |
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Keywords
- Exosome
- Human milk
- In vitro digestion
- Intestine
- MicroRNA
ASJC Scopus subject areas
- Biotechnology
- Food Science
Cite this
Human milk exosomes and their microRNAs survive digestion in vitro and are taken up by human intestinal cells. / Liao, Yalin; Du, Xiaogu; Li, Jie; Lönnerdal, Bo.
In: Molecular Nutrition and Food Research, Vol. 61, No. 11, 1700082, 01.11.2017.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Human milk exosomes and their microRNAs survive digestion in vitro and are taken up by human intestinal cells
AU - Liao, Yalin
AU - Du, Xiaogu
AU - Li, Jie
AU - Lönnerdal, Bo
PY - 2017/11/1
Y1 - 2017/11/1
N2 - Scope: Human milk exosomes provide a natural means of genetic material transfer to infants; however, the effect of gastric/pancreatic digestion milk exosomes stability and their microRNA content is largely unknown. Methods and results: We took a simulated gastric/pancreatic digestion protocol to perform in vitro digestion of milk exosomes, explore intestinal epithelial uptake, and further elucidate microRNA responses to digestion at early-, mid-, late lactation by massive parallel sequencing. Both undigested and digested exosomes enter human intestinal crypt-like cells (HIEC), with evidence of nuclear localization. We identified 288 mature microRNAs from all 24 exosome samples, and an additional 610 at low abundance. A large number of synapse development- and immune-related microRNAs were identified. hsa-miR-22-3p was the most abundant microRNA, and the top 15 microRNAs contributed ∼11% of the sequencing reads. Upon digestion, the overall microRNA abundance in human milk exosomes was stable. Conclusion: Our results for the first time reveal the survivability and complexity of human milk exosome microRNAs upon simulated gastric/pancreatic digestion, and the dynamics during lactation stages. The results suggest a previously underexplored area of infant response to genetic material in human milk exosomes.
AB - Scope: Human milk exosomes provide a natural means of genetic material transfer to infants; however, the effect of gastric/pancreatic digestion milk exosomes stability and their microRNA content is largely unknown. Methods and results: We took a simulated gastric/pancreatic digestion protocol to perform in vitro digestion of milk exosomes, explore intestinal epithelial uptake, and further elucidate microRNA responses to digestion at early-, mid-, late lactation by massive parallel sequencing. Both undigested and digested exosomes enter human intestinal crypt-like cells (HIEC), with evidence of nuclear localization. We identified 288 mature microRNAs from all 24 exosome samples, and an additional 610 at low abundance. A large number of synapse development- and immune-related microRNAs were identified. hsa-miR-22-3p was the most abundant microRNA, and the top 15 microRNAs contributed ∼11% of the sequencing reads. Upon digestion, the overall microRNA abundance in human milk exosomes was stable. Conclusion: Our results for the first time reveal the survivability and complexity of human milk exosome microRNAs upon simulated gastric/pancreatic digestion, and the dynamics during lactation stages. The results suggest a previously underexplored area of infant response to genetic material in human milk exosomes.
KW - Exosome
KW - Human milk
KW - In vitro digestion
KW - Intestine
KW - MicroRNA
UR - http://www.scopus.com/inward/record.url?scp=85027875352&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85027875352&partnerID=8YFLogxK
U2 - 10.1002/mnfr.201700082
DO - 10.1002/mnfr.201700082
M3 - Article
AN - SCOPUS:85027875352
VL - 61
JO - Molecular Nutrition and Food Research
JF - Molecular Nutrition and Food Research
SN - 1613-4125
IS - 11
M1 - 1700082
ER -