Human lysozyme expressed in the mammary gland of transgenic dairy goats can inhibit the growth of bacteria that cause mastitis and the cold-spoilage of milk

Elizabeth A. Maga, James S Cullor, Wayne Smith, Gary B. Anderson, James D. Murray

Research output: Contribution to journalArticle

66 Citations (Scopus)

Abstract

The addition of human milk components with intrinsic antimicrobial activity to livestock milk by genetic engineering has the potential to benefit milk safety and production as well as the health of the lactating animal. As a model for the dairy cow, we generated transgenic goats that expressed human lysozyme in their milk at 68% of the levels found in human milk. Milk from these transgenic animals had a bacteriostatic effect on both in vitro and in vivo growth of several microorganisms important to the dairy industry. In vitro, milk from transgenic animals was capable of slowing the growth of mastitis-causing strains of Escherichia coli (P < 0.02) and Staphylococcus aureus (P < 0.05) as well as the cold-spoilage organism Pseudomonas fragi (P < 0.02). The growth of an organism involved in cheese-making, Lactococcus lactis, was not affected by the presence of lysozyme in milk. The supplementation of control milk with purified lysozyme did not achieve the same inhibitory effect as milk from transgenic animals. In vivo, milk from transgenic animals supported less bacterial growth than control milk. This transgenic model demonstrates the possibilities offered by genetic engineering to enhance the antimicrobial nature of milk and the udder.

Original languageEnglish (US)
Pages (from-to)384-392
Number of pages9
JournalFoodborne Pathogens and Disease
Volume3
Issue number4
DOIs
StatePublished - Dec 2006

Fingerprint

Mastitis
dairy goats
Human Mammary Glands
Muramidase
lysozyme
spoilage
Goats
mammary glands
mastitis
Milk
genetically modified organisms
Bacteria
milk
bacteria
Growth
transgenic animals
Genetically Modified Animals
Genetic Engineering
Human Milk
breast milk

ASJC Scopus subject areas

  • Microbiology
  • Applied Microbiology and Biotechnology
  • Infectious Diseases
  • Food Science

Cite this

Human lysozyme expressed in the mammary gland of transgenic dairy goats can inhibit the growth of bacteria that cause mastitis and the cold-spoilage of milk. / Maga, Elizabeth A.; Cullor, James S; Smith, Wayne; Anderson, Gary B.; Murray, James D.

In: Foodborne Pathogens and Disease, Vol. 3, No. 4, 12.2006, p. 384-392.

Research output: Contribution to journalArticle

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